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Protocols
- Cell Clonogenic Survival Assay (The Science Advisory Board)
For testing the capability of adherent cells to survive and replicate following insult with chemicals or radiation.
http://www.scienceboard.net/resources/protocols.as...
Added: Thu Feb 19 2009, Hits: 7393, Reviews: 0 Write review Cached - Soft Agar Assay (Wallert and Provost Lab, Minnesota State University Moorhead)
The Soft Agar Assay for Colony Formation is an anchorage independent growth assay in soft agar, which is considered the most stringent assay for detecting malignant transformation of cells. For this assay, cells (pretreated with carcinogens or carcinogen inhibitors) are cultured with appropriate controls in soft agar medium for 21-28 days. Following this incubation period, formed colonies can either be analyzed morphologically using cell stain and quantifying the number of colonies formed per well.
http://web.mnstate.edu/provost/Soft%20Agar%20Assay...
Added: Sat Sep 29 2012, Hits: 2110, Reviews: 0 Write review -
Anchorage Independence Assay using Methyl Cellulose
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Mon Feb 02 2009, Hits: 1383, Reviews: 0 Write review Cached -
Hematopoietic Colony Forming Cell Assays
(Fero Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/fero/Protocols/Hematopoietic...
Added: Sun Aug 01 2010, Hits: 891, Reviews: 0 Write review Cached -
Noble Agar Assay
(The Science Advisory Board)
Noble agar is used to coat the tissue culture plates to cover the polylysine coating of the plate. On this noble agar coated, anchorage independent tissue culture plates normal and cancer cells will be seeded in very low density to prevent the contact between cells. Plating efficiency is a measure of the clonogenic ability of cells and Cancer cells will have higher plating efficiencies than normal cells.
http://www.scienceboard.net/resources/protocols.as...
Added: Wed Feb 18 2009, Hits: 1440, Reviews: 0 Write review Cached -
Soft Agar Assay
(Wallert and Provost Lab, Minnesota State University Moorhead)
http://www.stewartlab.net/Protocols/Soft_agar_1.ht...
Added: Thu May 27 2004, Hits: 2231, Reviews: 0 Write review Cached -
Soft Agar Assay
(UAB)
http://homepage.uab.edu/wzhang/SoftAgarAssay.html
Added: Thu Jun 03 2010, Hits: 755, Reviews: 0 Write review Cached -
Soft Agar Assay for Colony Formation
(BioProtocol)
http://www.bio.com/protocolstools/protocol.jhtml?i...
Added: Thu May 27 2004, Hits: 2798, Reviews: 0 Write review Cached -
Soft Agar Assay For Colony Formation
(Botwell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 817, Reviews: 0 Write review Cached -
Soft Agar Assay for Colony Formation
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Mon Feb 02 2009, Hits: 2498, Reviews: 0 Write review Cached -
Soft Agar Colony Formation Assay
(Arispe Lab, UCLA)
This assay is designed to assay a cell's ability to grow unattached to a surface and therefore suspended in agar.
http://www.mcdb.ucla.edu/Research/Arispe/Protocols...
Added: Thu Feb 19 2009, Hits: 3175, Reviews: 0 Write review