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- Cell Cytotoxicity Assay (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatments). Large amounts of label is released into the supernatant upon disruption of membrane integrity by the killing process and can be measured in a gamma-counter.
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Mon Oct 28 2002, Hits: 2691, Reviews: 0 Write review Cached -
3H Thymidine-Incorporation Assay for Rat-1a cells
(iProtocol)
http://iprotocol.mit.edu/protocol/128.htm
Added: Mon Dec 09 2002, Hits: 1385, Reviews: 0 Write review Cached -
3H-Thymidine Uptake by Cultured Cells
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 2949, Reviews: 0 Write review Cached -
3H-Thymidine Uptake by Cultured Cells
(iProtocol)
A radioactive method for measuring cultured cell growth with 3H-thymidine.
http://iprotocol.mit.edu/protocol/56.htm
Added: Mon Dec 09 2002, Hits: 1627, Reviews: 0 Write review Cached -
96-Well Cell Proliferation Assay
(Promega)
Colorimetric method for determining the number of viable cells in proliferation or chemosensitivity assays.
http://www.promega.com/tbs/tb169/tb169.html#iv
Added: Tue May 14 2002, Hits: 5194, Reviews: 0 Write review Cached -
Cell Proliferation Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Cell proliferation assay by cell counting.
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Mon Oct 28 2002, Hits: 3394, Reviews: 0 Write review Cached -
Cell Viability Assay
(http://www.hallym.ac.kr/~gene)
Dye exclusion method
http://www.hallym.ac.kr/~gene/MoleGene/protocol/ki...
Added: Tue May 14 2002, Hits: 4887, Reviews: 0 Write review Cached -
Crystal Violet Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay, crystal violet, stains DNA. Upon solubilization, the amount of dye taken up by the monolayer can be quantitated in a spectrophotometer or plate reader.
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Mon Oct 28 2002, Hits: 3133, Reviews: 0 Write review Cached -
Cytotoxicity Assays Protocol
(Hedrick Lab, UCSD Cancer Center)
Cell-mediated cytotoxicity is determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted and resuspended in 100 mCi of Na51Cr...
http://hedricklab.ucsd.edu/Protocol/cyto.html
Added: Tue Dec 10 2002, Hits: 2912, Reviews: 0 Write review Cached -
Detection of BrdU Incorportation in DNA Synthesizing Cells
(BD PharMingen)
A standard protocol for detecting DNA in cells.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 3364, Reviews: 0 Write review Cached