Growing More Cells: A Simple Guide to Small Volume Cell Culture Scale-Up
(Corning) Life science researchers are constantly being asked to grow more cells for cell-based experiments and assays, and producing recombinant proteins, antibodies, vaccines and viral vectors. There are a wide variety of products and solutions for scaling up cell cultures to meet these requirements. This 16-page guide will help you select the vessels and techniques that can best meet your needs for growing large amounts of cells (up to 1010cells) or producing more products from these cells in a typical research laboratory setting.
* Topics covered include: Advantages and disadvantages of the different vessels and systems used for growing large amounts of cells
* How to select the best culture system for your needs
* How to increase your chances of success
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Trypsinizing and Subculturing Mammalian Cells
(Journal of Visualized Experiments) As cells reach confluency, they must be subcultured or passaged. Failure to subculture confluent cells results in reduced mitotic index and eventually in cell death. The first step in subculturing is to detach cells from the surface of the primary culture vessel by trypsinization or mechanical means. The resultant cell suspension is then subdivided, or reseeded, into fresh cultures. Secondary cultures are checked for growth and fed periodically, and may be subsequently subcultured to produce tertiary cultures. The time between passaging of cells varies with the cell line and depends on the growth rate. http://www.jove.com/index/details.stp?ID=755 Added: Wed Feb 11 2009, Hits: 2668, Reviews: 0Write reviewCached