Protocols
- Single Cell Cloning by Serial Dilution
(Corning Life Science)
This 3-page protocol describes a simple approach using a 96 well plate to isolate colonies derived from single cells.
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 3886, Reviews: 0 Write review -
Preparation of Lymphocyte Cell Pellet for Storage
(Donis-Keller lab)
Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc5.ht...
Added: Tue May 14 2002, Hits: 869, Reviews: 0 Write review Cached -
Trypsinization of Adherent Cells
(Suprya Jayadev)
http://www.musc.edu/bcmb/ceramide/protocols/0005.h...
Added: Tue May 14 2002, Hits: 4056, Reviews: 0 Write review Cached -
Trypsinizing Cells
(Sefton Lab, Salk Institute)
http://pingu.salk.edu/~sefton/Hyper_protocols/tryp...
Added: Tue May 14 2002, Hits: 3789, Reviews: 0 Write review Cached -
Use of Cloning Cylinders for Harvesting Cell Colonies
(Corning Life Science)
3-page protocol that describes how to isolate and develop a population of cells (a clone) that are the descendants of a single cell.
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 2754, Reviews: 0 Write review