Protocols
- Cell Cloning by Serial Dilution 96 Well Plates
(Corning)
This 3-page protocol describes a simple approach using a 96 well plate to isolate colonies derived from single cells.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 3464, Reviews: 0 Write review -
Preparation of Lymphocyte Cell Pellet for Storage
(Donis-Keller lab)
Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc5.html
Added: Tue May 14 2002, Hits: 644, Reviews: 0 Write review Cached -
Stable Transfection and Cloning Harvesting
(Cell Biology Lab, School of Anatomy, UNSW, Sydney, Australia)
It describes in great detail how to pick up clones using a cloning cylinder, along with general transfection procedures.
http://anatomy.med.unsw.edu.au/cblonly/methods/mh1...
Added: Thu Apr 08 2004, Hits: 3979, Reviews: 0 Write review Cached -
Trypsinization of Adherent Cells
(Suprya Jayadev)
http://www.musc.edu/bcmb/ceramide/protocols/0005.h...
Added: Tue May 14 2002, Hits: 2821, Reviews: 0 Write review Cached -
Trypsinizing Cells
(Sefton Lab, Salk Institute)
http://pingu.salk.edu/~sefton/Hyper_protocols/tryp...
Added: Tue May 14 2002, Hits: 2449, Reviews: 0 Write review Cached -
Use of Cloning Cylinders for Harvesting Cell Colonies
(Corning)
3-page protocol that describes how to isolate and develop a population of cells (a clone) that are the descendants of a single cell.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 2163, Reviews: 0 Write review