Protocols
- Subculturing Monolayer Cell Cultures
(Corning Life Science)
This 5-page protocol provides a detailed procedure for subculturing attached cells using enzymatic dissociation. It explains the theory behind some of the keys steps in the process
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 7352, Reviews: 0 Write review -
Culture of BEND Cells (Bovine Endometrial Cells)
(Peter J. Hansen)
http://www.animal.ufl.edu/hansen/protocols/bend.ht...
Added: Tue May 14 2002, Hits: 512, Reviews: 0 Write review Cached -
Culture of endometrial explants
(Peter J. Hansen)
http://www.animal.ufl.edu/hansen/protocols/endocul...
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Lymphoblastoid Cell Lines from Frozen Whole Blood
(Donis-Keller lab)
Blood Samples can be stored frozen as a backup in case an LCL is needed at a later date.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc10.h...
Added: Tue May 14 2002, Hits: 904, Reviews: 0 Write review Cached -
Lymphocyte Transformation
(Donis-Keller lab)
Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque. During centrifugation, erythrocytes and granulocytes are aggregated by ficoll and rapidly settle to the bottom of the tube; lymphocytes and other mononuclear cells remain at the plasma-histopaque interface. Erythrocyte contamination is neglible. Most extraneous platelets are removed by low speed centrifugation during the washing steps.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc2.ht...
Added: Tue May 14 2002, Hits: 1686, Reviews: 0 Write review Cached -
Maintaining Lymphoblastoid Cell Lines
(Donis-Keller lab)
To grow lymphoblastoid cells for permanent storage and for DNA extraction.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc9.ht...
Added: Tue May 14 2002, Hits: 1377, Reviews: 0 Write review Cached - Maintenance of Cell Culture ( Contributed by Nanci Donacki )
Detailed procedure for culture and subculture cell in flask and plates
Added: Tue May 14 2002, Reviews: 0 Write review -
Preparation of Lymphoblastoid Cell Lines for Long-Term Storage
(Donis-Keller lab)
To store cell lines in a form that will insure recovery with high viability. A culture in logarithmic phase of growth with a total volume of 80-100 ml/T-75 flask should yield enough cells to freeze 10 ampules (1.0 ml/ampule). Cells should have a count of 4 X 106 cells/ampule to 9 X 106 cells/ampule. Too high or too low a cell count lowers recovery viability. Cell are frozen in RPMI-1640 with 15% Fetal Bovine Serum + 10% DMSO. Cultures are frozen slowly using a Model 700 Controller freezing chamber. This precision electronic device automatically controls the injection of liquid nitrogen into the freezing chamber to provide a 1 degrees C/minute freezing rate from +4 degrees C to -45 degrees C (with automatic heat of fusion compensation), then a 10 degrees C per minute freezing rate to -90 degrees C. Frozen ampules should be stored in liquid nitrogen for long term storage or in a -135 degrees C Cryopreservation System. Note: Cryotubes should be labeled with cell line number anddate prior to beginning this procedure.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc3.ht...
Added: Tue May 14 2002, Hits: 1203, Reviews: 0 Write review Cached -
Subculturing Cells
(Yu-li Wang's Lab, University of Massachusetts Medical School )
Basic procedures for routine cell culture
http://ylwang.umassmed.edu/protocol/cc/subcultu.ht...
Added: Fri Oct 17 2003, Hits: 4668, Reviews: 0 Write review Cached -
Transfer of Eukaryote Suspension Cultures
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
Added: Tue May 14 2002, Hits: 869, Reviews: 0 Write review Cached