Summary: Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. In DNA laddering assay, small fragments of oligonucleosomal DNA is extracted selectively from the cells whereas the higher molecular weight DNA stays associated with the nuclei. The isolated DNA is separated by electrophoresis and visualized using ethidium bromide.
Protocols
- Qualitative Analysis of DNA Fragmentation by Agarose Gel Electrophoresis (Paola Bossů Dompé Research Center, L'Aquila, Italy)
Formation of DNA fragments of oligonucleosomal size (180-200 bp) is an hallmark of apoptosis in many cell types. This protocol provides a method for DNA separation of fragmented and intact DNA fractions and for their analysis by agarose gel electrophoresis.
http://www.immunologia.unimore.it/SITO%20AC/APOBOO...
Added: Sat Jan 31 2009, Hits: 6923, Reviews: 0 Write review Cached -
DNA Laddering Assay
(Steven Finkbeiner, UCSF)
http://www.gladstone.ucsf.edu/gladstone/files/fink...
Added: Fri Feb 20 2009, Hits: 7205, Reviews: 0 Write review -
DNA Laddering Assay for Treated Cells
(CellDeath.de)
A convenient and successful method to detect DNA laddering in cells undergoing apoptosis
http://www.celldeath.de/apometh/dnaladd.html
Added: Sat Jan 31 2009, Hits: 5684, Reviews: 0 Write review Cached