Top : Cell Biology : Apoptosis : DNA Laddering Assay

Summary: Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. In DNA laddering assay, small fragments of oligonucleosomal DNA is extracted selectively from the cells whereas the higher molecular weight DNA stays associated with the nuclei. The isolated DNA is separated by electrophoresis and visualized using ethidium bromide.