1.2 | Apoptosis Assay Methods | 6 |
| Method/Product selection guide | 7 |
1.2.1 | Methods for studying apoptosis in cell populations | 8 |
1.2.1.1 | Assays that measure DNA fragmentation | 8 |
| Apoptotic DNA Ladder Kit | 11 |
| Cell Death Detection ELISAPLUS | 13 |
1.2.1.2 | Assays that measure apoptosis-induced proteases (caspase) | 16 |
| Caspase 3 Activity Assay | 17 |
| Anti-PARP | 20 |
1.2.1.3 | Summary of methods for studying apoptosis in cell populations | 22 |
1.2.2 | Methods for studying apoptosis in individual cells | 24 |
1.2.2.1 | The TUNEL enzymatic labeling assay | 24 |
| In Situ Cell Death Detection Kit, Fluorescein | 27 |
| In Situ Cell Death Detection Kit, AP | 29 |
| In Situ Cell Death Detection Kit, POD | 29 |
1.2.2.2 | Assays that measure membrane alterations | 31 |
| Annexin-V-FLUOS | 32 |
| Annexin-V-FLUOS Staining Kit | 32 |
| Annexin-V-AlexaTM 568 | 32 |
| Annexin-V-Biotin | 34 |
1.2.2.3 | Assays that use DNA stains | 36 |
| DAPI, Ethidium bromide, Propidium iodide | 36 |
1.2.2.4 | Summary of methods for studying apoptosis in individual cells | 38 |
1.2.3 | Detection of apoptosis-related proteins | 40 |
| Anti-Fas (CD95/Apo-1) | 41 |
| Anti-Fas-Biotin (CD95/Apo-1) | 42 |
| Anti-Bcl-2 oncoprotein, human | 44 |
| Anti-p53 Protein, mutant | 46 |
| Anti-p53 Protein pan | 46 |
| Anti-p53 pan | 46 |
| Anti-p53, Biotin labeled | 46 |
| Anti-p53, Peroxidase labeled | 46 |
| p53pan ELISA | 48 |
1.3 | Cytotoxicity Assay Methods | 50 |
1.3.1 | Relationship between cytotoxicity, apoptosis, and necrosis | 50 |
1.3.2 | Methods for studying cytotoxicity | 50 |
1.3.2.1 | Assays the measure plasma membrane leakage | 51 |
| Cytotoxicity Detection Kit (LDH) | 52 |
| Cellular DNA Fragmentation ELISA | 54 |
1.3.2.2 | Assays that measure metabolic activity | 58 |
| Cell Proliferation Kit I (MTT) | 58 |
| Cell Proliferation Kit II (XTT) | 58 |
| Cell Proliferation Reagent WST-1 | 58 |
1.3.2.3 | Summary of methods for studying cytotoxicity | 60 |
2.2 | Cell proliferation/viability assay methods | 67 |
| Method/Product selection guide | 68 |
2.2.1 | Methods for studying cell proliferation and viability in cell populations | 70 |
2.2.1.1 | Assays that measure metabolic activity | 70 |
| Cell Proliferation Kit I (MTT) | 73 |
| Cell Proliferation Kit II (XTT) | 74 |
| Cell Proliferation Reagent WST-1 | 75 |
2.2.1.2 | Assays that measure DNA synthesis | 77 |
| BrdU Labeling and Detection Kit III | 79 |
| Cell Proliferation ELISA, BrdU (colorimetric) | 81 |
| Cell Proliferation ELISA, BrdU (chemiluminescence) | 81 |
2.2.1.3 | Summary of methods for studying cell proliferation and cell viability in cell populations | 84 |
2.2.1.4 | Single reagents for the measurement of DNA synthesis | 84 |
2.2.2 | Methods for studying cell proliferation and viability in individual cells | 86 |
2.2.2.1 | Assays that measure DNA synthesis | 86 |
| BrdU Labeling and Detection Kit I | 87 |
| BrdU Labeling and Detection Kit II | 87 |
| BrdU Labeling and Detection Kit, FLUOS | 88 |
| In situ Cell Proliferation Kit, FLUOS | 90 |
| In Situ Cell Proliferation Kit, AP | 93 |
| Anti-BrdU, formalin grade | 93 |
| Anti-BrdU-Fluorescein | 93 |
| Anti-BrdU-AP, F(ab)2 fragments | 93 |
| Anti-BrdU-Peroxidase, Fab fragment | 93 |
2.2.2.2 | Assays that monitor expression of cell cycle-associated antigens | 96 |
| Monoclonal antibodies to cell cycle-associated antigens | 97 |
2.2.2.3 | Summary of methods for studying cell proliferation and
|