Subcategories
Protocols
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Assay of superoxide dismutase activity by combining electrophoresis and densitometry
(Ching-Nen Chen and Shu-Mei Pan, Department of Botany, National Taiwan University, Taipei)
This paper describes a modified technique was developed to assay superoxide dismutase (SOD) activity by combining polyacrylamide gel electrophoresis and densitometry
http://ejournal.sinica.edu.tw/bbas/content/1996/2/...
Added: Mon Sep 23 2002, Hits: 2101, Reviews: 0 Write review Cached -
Cellulase Assays
(OSMOWEB, SAPS, UK )
Two possible protocols are described, a gel diffusion assay method and a viscosity reduction assay method.
http://www-saps.plantsci.cam.ac.uk/osmoweb/cellula...
Added: Thu Oct 03 2002, Hits: 1158, Reviews: 0 Write review Cached -
Detection of D-Amino-Acid Oxidase
(Biological Procedures Online )
Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
http://www.biologicalprocedures.com/bpo/arts/1/7/m...
Added: Wed Sep 25 2002, Hits: 879, Reviews: 0 Write review Cached -
Enzyme Kinetics Assay
(Juergen Reichardt's Lab, Institute for Genetic Medicine, University of Southern California)
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution.
http://www-scf.usc.edu/~fctsai/reichardt/protocols...
Added: Sat Oct 12 2002, Hits: 578, Reviews: 0 Write review Cached -
Glucosamine Rapid Assay
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 651, Reviews: 0 Write review Cached -
Measurement of a Bacterial Enzyme Activity in Cell Lysates
(Biological Procedures Online )
Three diffirent methods: nuclear magnetic resonance spectroscopy, radioactive tracer analysis, and spectrophotometry are used to detect the kinetic characteristics and regulation of aspartate carbamoyltransferase activity in lysates and cell extracts of Helicobacter pylori by
http://www.biologicalprocedures.com/bpo/arts/1/5/m...
Added: Wed Sep 25 2002, Hits: 1195, Reviews: 0 Write review Cached -
Method for Assaying Deubiquitinating Enzymes
(Biological Procedures Online )
A general method for the assay of deubiquitinating enzymes is described in detail using 125I-labeled ubiquitin-fused alpha NH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate
http://www.biologicalprocedures.com/bpo/arts/1/11/...
Added: Wed Sep 25 2002, Hits: 665, Reviews: 0 Write review Cached -
Pectinase Assay
(OSMOWEB, SAPS, UK )
The principle of this assay depends upon measuring the amount of watery juice released from tinned apple puree (which is very rich in pectin) as a result of pectinase action.
http://www-saps.plantsci.cam.ac.uk/osmoweb/pectina...
Added: Thu Oct 03 2002, Hits: 954, Reviews: 0 Write review Cached -
Polygalacturonase Assay
(OSMOWEB, SAPS, UK )
The principle of this assay is to mix an extract of fruit (or vegetable) with a viscous solution of polygalacturonic acid, and then note the decrease in viscosity due to its breakdown by the enzyme.
http://www-saps.plantsci.cam.ac.uk/osmoweb/polygas...
Added: Thu Oct 03 2002, Hits: 592, Reviews: 1 Read reviews Write review Cached -
Polyphenoloxidase (Catechol Oxidases) Assay
(OSMOWEB, SAPS, UK )
In this technique, the change from a colourless solution of catechol to coloured benzoquinone is followed with a colorimeter. A fruit extract is added to a solution of catechol and the rate of formation of coloured benzoquinone is measured. The faster the rate of increase in absorbance of the reaction mixture, the greater the polyphenoloxidase activity of the fruit extract.
http://www-saps.plantsci.cam.ac.uk/osmoweb/ppo.htm
Added: Thu Oct 03 2002, Hits: 585, Reviews: 0 Write review Cached -
Protease Assay
(OSMOWEB, SAPS, UK )
Protease enzymes are added to a milky colloidal suspension of egg albumen. As the protease enzymes digest the suspended particles of proteins, the mixture becomes more transparent. The absorbance changes in the reaction mixture are followed with a colorimeter. The protease content, for example in extracts of fruits, can be assayed by measuring the rate at which the solution of egg albumen and extract becomes clearer.
http://www-saps.plantsci.cam.ac.uk/osmoweb/proteas...
Added: Thu Oct 03 2002, Hits: 1635, Reviews: 0 Write review Cached