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Protocols
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Assay of superoxide dismutase activity by combining electrophoresis and densitometry
(Ching-Nen Chen and Shu-Mei Pan, Department of Botany, National Taiwan University, Taipei)
This paper describes a modified technique was developed to assay superoxide dismutase (SOD) activity by combining polyacrylamide gel electrophoresis and densitometry
http://ejournal.sinica.edu.tw/bbas/content/1996/2/...
Added: Mon Sep 23 2002, Hits: 3668, Reviews: 0 Write review Cached -
Cellulase Assays
(OSMOWEB, SAPS, UK )
Two possible protocols are described, a gel diffusion assay method and a viscosity reduction assay method.
http://www-saps.plantsci.cam.ac.uk/osmoweb/cellula...
Added: Thu Oct 03 2002, Hits: 2210, Reviews: 0 Write review Cached -
Detection of D-Amino-Acid Oxidase
(Biological Procedures Online )
Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
http://www.biologicalprocedures.com/bpo/arts/1/7/m...
Added: Wed Sep 25 2002, Hits: 1592, Reviews: 0 Write review Cached -
Enzyme Activity Assays for cyanide-degrading Nitrilases
(South African Structural Biology Initiative)
For activity assays of cyanide-degrading Nitrilases and nitrilases that hydrolyze higher nitriles (benzonitrile among others) resulting in the production of ammonia among other products.
http://sbio.uct.ac.za/Sbio/documentation/Enzymes%2...
Added: Wed Jan 18 2006, Hits: 424, Reviews: 0 Write review Cached -
Enzyme Kinetics Assay
(Juergen Reichardt's Lab, Institute for Genetic Medicine, University of Southern California)
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution.
http://www-scf.usc.edu/~fctsai/reichardt/protocols...
Added: Sat Oct 12 2002, Hits: 2232, Reviews: 0 Write review Cached -
Glucosamine Rapid Assay
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 964, Reviews: 0 Write review Cached -
Measurement of a Bacterial Enzyme Activity in Cell Lysates
(Biological Procedures Online )
Three diffirent methods: nuclear magnetic resonance spectroscopy, radioactive tracer analysis, and spectrophotometry are used to detect the kinetic characteristics and regulation of aspartate carbamoyltransferase activity in lysates and cell extracts of Helicobacter pylori by
http://www.biologicalprocedures.com/bpo/arts/1/5/m...
Added: Wed Sep 25 2002, Hits: 2804, Reviews: 0 Write review Cached -
Method for Assaying Deubiquitinating Enzymes
(Biological Procedures Online )
A general method for the assay of deubiquitinating enzymes is described in detail using 125I-labeled ubiquitin-fused alpha NH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate
http://www.biologicalprocedures.com/bpo/arts/1/11/...
Added: Wed Sep 25 2002, Hits: 1448, Reviews: 0 Write review Cached -
NBT Assay - Brice Weinberg Protocol
(Hannun/Obeid Lab, Duke Univ.)
http://www.duke.edu/web/ceramide/protocols/0014.ht...
Added: Sun Apr 24 2005, Hits: 1138, Reviews: 0 Write review Cached -
Nitroblue Tetrazolium (NBT) Assay
(Hannun/Obeid Lab, Duke Univ.)
http://www.duke.edu/web/ceramide/protocols/0013.ht...
Added: Sun Apr 24 2005, Hits: 1459, Reviews: 0 Write review Cached