Top : Molecular Biology : RNA : microRNA : microRNA Cloning

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Protocols
  • miRNA Cloning Protocol (Fatih Kocabas, Moleküler Biyolog ve Genetikçi, UT Southwestern Medical Center at Dallas)
    http://fatihkocabas.net/mirnacloning.aspx
    Added: Sat Jan 31 2009, Hits: 3216, Reviews: 0  Write review   Cached
  • MicroRNA (miRNA) and siRNA Cloning Protocol PDF file (Bartel Lab, MIT)
    This protocol consists of the following procedures: 1. Oligo Design; 2. ImpA Synthesis; 3. Synthesizing Oligos; 4. Oligonucleotide Adenylation; 5. Small RNA Purification; 6. 3' Adaptor Ligation; 7. 5' Adaptor Ligation; 8. RT-PCR; 9. Concatamerization; 10. Cloning into Topo vector; 11. Screen and Sequence
    http://web.wi.mit.edu/bartel/pub/protocols/miRNACl...
    Added: Sat Jan 31 2009, Hits: 3665, Reviews: 0  Write review  
  • Theory, principles and background info MicroRNA Cloning Overview (Ambion)
    This overview gives you a general idea on how miR is cloned starting from small RNA isolation and purification.
    http://www.ambion.com/techlib/tn/123/6.html
    Added: Sat Jan 31 2009, Hits: 2484, Reviews: 0  Write review   Cached