microRNA/microRNA Cloning Protocols

microRNA (miRNA) Cloning Protocol (Ambros Lab at Dartmouth Medical School)
miRNA is first ligated to linkers and reverse transcribed. The resulted cDNA is then PCR amplified and purified for inserting into cloning vector.
http://banjo.dartmouth.edu/lab/MicroRNAs/Ambros_mi...
Added: Thu Jun 09 2005, Hits: 3396, Reviews: 0 Write review Cached
MicroRNA (miRNA) and siRNA Cloning Protocol (Bartel Lab, MIT)
This protocol consists of the following procedures: 1. Oligo Design; 2. ImpA Synthesis; 3. Synthesizing Oligos; 4. Oligonucleotide Adenylation; 5. Small RNA Purification; 6. 3' Adaptor Ligation; 7. 5' Adaptor Ligation; 8. RT-PCR; 9. Concatamerization; 10. Cloning into Topo vector; 11. Screen and Sequence
http://web.wi.mit.edu/bartel/pub/protocols/miRNACl...
Added: Thu Jun 09 2005, Hits: 3057, Reviews: 0 Write review
MicroRNA Cloning Overview (Ambion)
This overview gives you a general idea on how miR is cloned starting from small RNA isolation and purification.
http://www.ambion.com/techlib/tn/123/6.html
Added: Sat Aug 27 2005, Hits: 2551, Reviews: 0 Write review Cached