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New Forum Archives (2009-): :
931. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
933. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
935. overloading GAPDH but nice band of interest - (reply: 2)
937. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
939. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
941. Native PAGE w. Western protein marker - (reply: 4)
943. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
945. changing colour of front dye in sds-page - (reply: 10)
947. how stable is alpha mouse HRP? - left on the bench (reply: 2)
949. Too many bands in my SDS-PAGE - (reply: 5)
951. Uneven bands in SDS-PAGE - (reply: 5)
953. Preparation of tree tissue for western blot - (reply: 1)
955. direct lysis in Laemmli buffer - (reply: 4)
957. NFkB western blotting - (reply: 3)
959. how long will a 1M DTT solution will last in -20C - (reply: 5)
933. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
935. overloading GAPDH but nice band of interest - (reply: 2)
937. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
939. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
941. Native PAGE w. Western protein marker - (reply: 4)
943. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
945. changing colour of front dye in sds-page - (reply: 10)
947. how stable is alpha mouse HRP? - left on the bench (reply: 2)
949. Too many bands in my SDS-PAGE - (reply: 5)
951. Uneven bands in SDS-PAGE - (reply: 5)
953. Preparation of tree tissue for western blot - (reply: 1)
955. direct lysis in Laemmli buffer - (reply: 4)
957. NFkB western blotting - (reply: 3)
959. how long will a 1M DTT solution will last in -20C - (reply: 5)