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31.
No or Super Strong Signal -
(reply: 4)
33.
Storing temperature for protein after SDS sample buffer and boiling? -
(reply: 2)
35.
Heating to denature and reduce protein: same temperature? -
(reply: 1)
37.
2 bands very close in the expected place -
(reply: 2)
39.
bacterial culture volume for recombinant protein to be detected in Western Blot -
(reply: 3)
41.
Non-specific binding of protein in western blot -
(reply: 3)
43.
More than 10 min exposure time in chemiluminescence detection -
(reply: 2)
45.
layer in between resolving and stacking gel -
(reply: 1)
47.
mRNA vs protein correlation issues, breaks central dogma -
(reply: 1)
49.
Insanely difficult time trying to cast large FACE gel for PROTEAN XI system -
(reply: 1)
51.
No band detected in WB -
(reply: 4)
53.
Western Blot diffuse bands -
(reply: 3)
55.
Bubbles in western blot gel "AFTER" running gel -
(reply: 2)
57.
Some bands transferred, some not..? Why??? -
(reply: 1)
59.
Heat Denaturation without added denaturant (SDS or Urea) -
(reply: 1)
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