Overview: Real-Time PCR primers sets may be designed using standard primer design algorithms without any modification. As with all PCR amplifications, however, the specific reaction conditions for each set must be optimized, particularly primer concentration, annealing temperature and magnesium chloride concentration.
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Real-Time PCR Primer Design (Genscript.com)
A free online tool for design real-time PCR primers and probes. The input is a GenBank accession number or a DNA sequence. Customized variables include primer or probe Tm, amplicon size, whether or not pick primer/probe crossing exon junction.
https://www.genscript.com/ssl-bin/app/primer
Added: Sun Jul 10 2005, Hits: 11175, Reviews: 0 Write review -
RTPrimerDB - Real-Time PCR Primer and Probe Database (Center for Medical Genetics, Ghent University Hospital)
a public database for primer and probe sequences used in real-time PCR assays employing popular chemistries (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon) to prevent time-consuming primer design and experimental optimisation, and to introduce a certain level of uniformity and standardisation among different laboratories
http://medgen.ugent.be/rtprimerdb/
Added: Sun Jul 10 2005, Hits: 4592, Reviews: 0 Write review Cached -
Beacon Designer Free Edition
(Premier Biosoft)
Beacon Designer free edition is an online tool that can be used to screen SYBRŽ Green primers and TaqManŽ probes for possible secondary structures such as dimers or hairpins.
http://www.premierbiosoft.com/qpcr/index.html
Added: Sun Sep 16 2007, Hits: 737, Reviews: 0 Write review Cached -
Guidelines for Designing Primers and Probes using Primer Express
(W.M. Keck Facility at Yale)
http://keck.med.yale.edu/affymetrix/rtpcr/design.h...
Added: Wed Jul 19 2006, Hits: 2583, Reviews: 0 Write review Cached -
Primer Bank
(Dr. Xiaowei Wang at the Department of Molecular Biology, Massachusetts General Hospital)
PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). There are several ways to search for primers: by GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). PrimerBank contains about 180,000 primers covering most known human and mouse genes.
http://pga.mgh.harvard.edu/primerbank/index.html
Added: Fri May 14 2004, Hits: 4986, Reviews: 0 Write review Cached -
Quantitative PCR Primer Database (QPPD)
(NCI-Frederick Gene Expression Laboratory and the CGAP Genome Annotation Initiative)
provides information about primers and probes that can be used to quantitate human and mouse mRNA by reverse transcription polymerase chain reaction (RTPCR) assays. All data has been gathered from published articles, cited in PubMed.
http://web.ncifcrf.gov/rtp/gel/primerdb/
Added: Wed Jul 19 2006, Hits: 1677, Reviews: 0 Write review Cached -
Real-Time PCR Primer and Probe Design Guidelines
(RRC Core Genomics Facility, University of Illinois at Chicago)
http://www.uic.edu/depts/rrc/cgf/realtime/primer.h...
Added: Sun Jul 10 2005, Hits: 4867, Reviews: 0 Write review -
Real-Time PCR Primer Database
(Steven W. Johnson, Ph.D.)
A frequently updated database of validated primer/probe sets for quantitation of a large number of genes of various organisams.
http://www.realtimeprimers.org/
Added: Sat Mar 27 2004, Hits: 6438, Reviews: 0 Write review Cached