- PCR Primers For Gene Expression Detection or Quantification

PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains about 180,000 primers covering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). 

The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency. Up to now, we have tested over a thousand primers and the design success rate is > 99%. 

Publication:

Xiaowei Wang and Brian Seed (2003) A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Research 31(24): e154; pp.1-8.

Send email to xwang@molbio.mgh.harvard.edu with questions or comments about this web site.
Last modified: April 12, 2004