DNA Extraction from Archival Formalin-fixed, Paraffin-embedded Tissue Sections
|Author: Shi et al.|
|Source: Contributed by APostodoc|
|Date Added: Thu May 06 2004|
|Date Modified: Thu May 06 2004|
|Abstract: Describes two methods of extracting DNA from archived, paraffin-embedded sections. One is the standard non-heating enzymatic digestion method; the other is heating method based on the principles of the antigen retrieval technique, which yields better DNA quantity.|
Section 1. Non-heating DNA Extraction Protocol
Section 2. Heating Protocol for DNA Extraction
According to Shi et al, heating before following the standard protocol above can get high DNA yield.
Materials and Reagents
Add 28.6 mM of each chemical
Adjust pH to 9 using 0.2 N sodium hydroxide
Shi et al, Journal of Histochemistry and Cytochemistry, Vol. 50, 1005-1011, August 2002.