Protocols
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β-Galactosidase Staining
(Cowin Lab, New York University)
Protocol for determining the percentage of cells expressing lacZ following transient or stable transfection of plasmids that contain lacZ
http://celljunctions.med.nyu.edu/lab/notprivate/me...
Added: Sat Mar 27 2010, Hits: 1984, Reviews: 0 Write review -
Assay for beta-galactosidase in E. Coli
(Biology, Hamline University)
This procedure is designed to induce and measure the level of ß-galactosidase in E. coli. Cells grown in the absence of lactose do not synthesize ß-galactosidase.
http://www.hamline.edu/depts/biology/courses/genet...
Added: Sun May 30 2004, Hits: 3186, Reviews: 0 Write review -
Beta-gal staining of eukaryotic cells in vitro
(Botwell Lab)
Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity.
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 282, Reviews: 0 Write review Cached -
Beta-Galactosidase Activity Assay
(John Roth, Division of Biological Sciences,University of California at Davis)
This assay is used to monitor transcription from insertion elements that encode the beta-galactosidase enzyme that have inserted into different genes.
http://rothlab.ucdavis.edu/protocols/beta-galactos...
Added: Sun May 30 2004, Hits: 4096, Reviews: 0 Write review Cached -
Beta-Galactosidase Enzyme Assay
(Promega)
For assaying beta-galactosidase activity in extracts prepared from cells transfected with beta-galactosidase reporter vectors.
http://www.promega.com/tbs/tb097/tb097.html
Added: Tue May 14 2002, Hits: 3788, Reviews: 0 Write review Cached -
Reporter Assays for Luciferase and ß-Galactosidase
(Shivdasani Lab, Harvard Medical School)
http://research.dfci.harvard.edu/shivdasani/protoc...
Added: Fri Apr 03 2009, Hits: 4373, Reviews: 0 Write review