Protocols
- Single-Cell Electroporation (Hollis Cline Lab, Cold Spring Harbor Laboratory)
Single-cell electroporation (SCE) is a technique to deliver genes into individual cells within intact tissues, although it may also be applicable to cells in disperse cultures. Targeting transcription to individual cells is achieved by restricting both the DNA and the electric field required for electroporation to the 0.6-1 µm tip of a glass micropipette. This technique is relatively easy to setup and perform, can yield high transfection rates, and requires relatively inexpensive, common laboratory equipment.
http://www.cshl.org/labs/cline/sce.html
Added: Wed Oct 22 2003, Hits: 1295, Reviews: 0 Write review Cached -
Transfection of Mammalian Cell (Electroporation Method)
(Botwell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 758, Reviews: 0 Write review Cached