Top : Molecular Biology : Transfection : Calcium Phosphate Transfection
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Protocols
  • Calcium Phosphate Transfection PDF file (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
    Describes transfection, clone picking and screening
    http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfe...
    Added: Wed Aug 14 2002, Hits: 5284, Reviews: 0  Write review  
  • Calcium Phosphate Transfection of Neurons in Primary Culture (Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
    http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
    Added: Tue Oct 01 2002, Hits: 409, Reviews: 0  Write review   Cached
  • CaPO3 Transfection (Cowin Lab, New York University)
    http://celljunctions.med.nyu.edu/lab/notprivate/me...
    Added: Sat Mar 27 2010, Hits: 555, Reviews: 0  Write review   Cached
  • CaPO4 Transfection for Chromaffin Cells (Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research)
    http://hypertension.ucsd.edu/list_of_protocols/15_...
    Added: Tue Feb 17 2004, Hits: 461, Reviews: 0  Write review   Cached
  • CaPO4 Transfection of Mammalian Cells (Laboratory of Dr. Bart Frank, Oklahoma Medical Research Foundation)
    http://www.omrf.org/omrf/research/09/FrankLab/CAPO...
    Added: Sat Jan 31 2009, Hits: 1942, Reviews: 0  Write review   Cached
  • Minitransfection: A protocol for transfecting HEK293 cells in individual wells of 384-well plates us (Daniel Gilbert, Lynch Lab, QLD Brain Institute, University of QLD, Australia)
    We describe a robust protocol for calcium phosphate-based transfection of HEK293 cells in 384-well plates. Cells in single wells were successfully transfected with up to three different plasmid DNAs (YFP-I152L, GlyR, GABAAR and TRPV1). Half-maximal transfection efficiency was achieved at a plasmid DNA concentration of 5 ng/well. Transfection rates were comparable to efficiencies obtained by conventional calcium phosphate-based transfection in 60 mm dishes. We were motivated to develop a method requiring low nanogram plasmid DNA quantities in order to make it possible to screen random mutant plasmid DNA libraries in a cost effective manner. The protocol is included as Supp Info to the publication at the provided URL. Please email j.lynch@uq.edu.au if you cannot access the protocol online.
    http://www.frontiersin.org/molecularneuroscience/p...
    Added: Sun Nov 15 2009, Hits: 522, Reviews: 0  Write review   Cached
  • Simplified Calcium Phosphate Coprecipitation (Fero Lab, Fred Hutchinson Cancer Research Center)
    http://labs.fhcrc.org/fero/Protocols/CaPO4_transfe...
    Added: Sat Jan 31 2009, Hits: 983, Reviews: 0  Write review   Cached
  • Transfection of Mammalian Cell (Calcium Phophate Method) (Botwell Lab)
    http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
    Added: Tue May 14 2002, Hits: 984, Reviews: 0  Write review   Cached