Protocols
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Calcium Phosphate Transfection
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Describes transfection, clone picking and screening
http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfe...
Added: Wed Aug 14 2002, Hits: 7602, Reviews: 0 Write review -
Calcium Phosphate Transfection of Neurons in Primary Culture
(Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
Added: Tue Oct 01 2002, Hits: 425, Reviews: 0 Write review Cached -
CaPO3 Transfection
(Cowin Lab, New York University)
http://celljunctions.med.nyu.edu/lab/notprivate/me...
Added: Sat Mar 27 2010, Hits: 1195, Reviews: 0 Write review Cached -
CaPO4 Transfection for Chromaffin Cells
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research)
http://hypertension.ucsd.edu/list_of_protocols/15_...
Added: Tue Feb 17 2004, Hits: 635, Reviews: 0 Write review Cached -
CaPO4 Transfection of Mammalian Cells
(Laboratory of Dr. Bart Frank, Oklahoma Medical Research Foundation)
http://www.omrf.org/omrf/research/09/FrankLab/CAPO...
Added: Sat Jan 31 2009, Hits: 2804, Reviews: 0 Write review Cached -
Minitransfection: A protocol for transfecting HEK293 cells in individual wells of 384-well plates us
(Daniel Gilbert, Lynch Lab, QLD Brain Institute, University of QLD, Australia)
We describe a robust protocol for calcium phosphate-based transfection of HEK293 cells in 384-well plates. Cells in single wells were successfully transfected with up to three different plasmid DNAs (YFP-I152L, GlyR, GABAAR and TRPV1). Half-maximal transfection efficiency was achieved at a plasmid DNA concentration of 5 ng/well. Transfection rates were comparable to efficiencies obtained by conventional calcium phosphate-based transfection in 60 mm dishes. We were motivated to develop a method requiring low nanogram plasmid DNA quantities in order to make it possible to screen random mutant plasmid DNA libraries in a cost effective manner. The protocol is included as Supp Info to the publication at the provided URL. Please email j.lynch@uq.edu.au if you cannot access the protocol online.
http://www.frontiersin.org/molecularneuroscience/p...
Added: Sun Nov 15 2009, Hits: 976, Reviews: 0 Write review Cached -
Simplified Calcium Phosphate Coprecipitation
(Fero Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/fero/Protocols/CaPO4_transfe...
Added: Sat Jan 31 2009, Hits: 1487, Reviews: 0 Write review Cached -
Transfection of Mammalian Cell (Calcium Phophate Method)
(Botwell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 1008, Reviews: 0 Write review Cached