Protocols
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ALF Sequencing with Fluorescein Labelled Nucleotides
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 109, Reviews: 0
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Deletion Mutagenesis
(Promega)
For construction of plasmid or M13 subclones containing progressive unidirectional deletions of inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation, transformation...
http://www.promega.com/tbs/tm006/tm006.html#i
Added: Tue May 14 2002, Hits: 62, Reviews: 0
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Dye Terminator Cycle Sequencing
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 288, Reviews: 0
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Nested Deletions
(Bowtell Lab)
Nested Deletions using exonuclease-III and mung bean nuclease
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 53, Reviews: 0
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Site-directed Mutagenesis
(Promega)
For generation and selection of oligonucleotide-directed mutants
http://www.promega.com/tbs/tm001/TM001.html#v
Added: Tue May 14 2002, Hits: 86, Reviews: 0
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