|Dye Terminator Cycle Sequencing |
STORE REACTION PREMIX AT -20°C (OCONNELL LAB).
THAW AND MAINTAIN ON ICE PRIOR TO USE
Add the following to a PCR tube:
All reactions in 20µl
Overlay mix with oil
25 cycles in total
(Program No.18- Bowtell lab PCR Machine)
Removal of Dye-Labelled Nucleotides Following Cycle Sequencing
Use Microspin G-50 columns.
Angela Higgins Automated DNA Analysis Facility School of Biochemistry and Molecular Biology University of New South Wales Sydney 2052
|Disclaimer: The following set of protocols were contributed by various members of our lab (past and present): Christine Andrews, Fiona Christensen, Neil Della, Ross Dickins, Debbie Donald, Andrew Holloway, Gary Hime, Colin House, Yinling Hu, Rachael Parkinson, Nadia Traficante, Hannah Robertson, Ping Fu and Dennis Wang. Special thanks to Vicki Hammond, Frank Kontgen and Maria Murphy, who contributed many of the ES cell protocols. Sections dealing with Photomicroscopy, Polyclonal and Monoclonal Antibody Production were provided by members of Gerry Rubin's Laboratory (Berkeley). Any comments in the methods (technical errors etc.) E-mail: firstname.lastname@example.org |
David Bowtell PMCI October 1998