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Protocols
- Avoiding Ribonucleases Contamination (NEB)
Why RNA is more susceptible to degradation than DNA and how to avoid it...
http://www.neb.com/nebecomm/tech_reference/general...
Added: Tue May 14 2002, Hits: 1190, Reviews: 0 Write review Cached - How to Maintain an RNase-free Lab (Ambion)
it is essential to develop a program of RNase control involving reagent preparation and testing, routine decontamination, and the use of certified RNase-free reagents and equipment such as microfuge tubes and pipette tips.
http://www.ambion.com/techlib/tb/tb_180.html
Added: Thu May 08 2003, Hits: 1303, Reviews: 0 Write review Cached -
Linear Polyacrylamide as Carrier in Ethanol Precipitation of Nucleic Acids
(Hideaki Shiraishi, Kyoto University)
Linear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with ethanol. Although glycogen is often used as carrier in ethanol precipitation, it has been shown that glycogen inhibits the activity of an ssDNA-binding protein on which linear polyacrylamide has no effect. Here is the method on how to prepare linear polyacrylamide used as carrier in ethanol precipitation of nucleic acids.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1529, Reviews: 0 Write review Cached -
Preparation of solutions and equipment for isolation of RNA
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/5-7-1.pdf
Added: Sat Aug 17 2002, Hits: 1548, Reviews: 0 Write review -
RNase Activity Staining on SDS-PAGE
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
http://ag.udel.edu/plsc/faculty/pamgreen/rnase.htm
Added: Sat Oct 25 2003, Hits: 620, Reviews: 0 Write review Cached -
RNase Control
(Ambion)
Pinpoints some of the sources of RNase contamination and highlights ways to eliminate contamination problems at various stages while working with RNA
http://www.ambion.com/techlib/basics/rnasecontrol/...
Added: Thu May 08 2003, Hits: 655, Reviews: 0 Write review Cached -
Solublization of RNA in Formamide
(NWFSC)
Resuspending RNA in Formamide has several benefits over storage in water or ethanol. First, formamide will protect RNA from RNases. The RNA is also extremely stable...
http://micro.nwfsc.noaa.gov/protocols/rnasoluble.h...
Added: Tue May 14 2002, Hits: 624, Reviews: 0 Write review -
Working with RNA
(Ambion)
Tips on handing nuclear acid during phenol extraction, ethanol precipitation.
http://www.ambion.com/techlib/tips/handling-rna.ht...
Added: Tue May 14 2002, Hits: 1612, Reviews: 0 Write review Cached