Related Categories:

• RNA Quantitation

Protocols

• How to Maintain an RNase-free Lab (Ambion)
  it is essential to develop a program of RNase control involving reagent preparation and testing, routine decontamination, and the use of certified RNase-free reagents and equipment such as microfuge tubes and pipette tips.
  http://www.ambion.com/techlib/tb/tb_180.html
  Added: Thu May 08 2003, Hits: 3782, Reviews: 0  Write review   Cached
• How to make DEPC-treated water and Tris Buffer (Qiagen)
  Solutions (water and other solutions) should be treated with 0.1% DEPC. DEPC is a strong, but not absolute, inhibitor of RNases. It is commonly used at a concentration of 0.1% to inactivate RNases on glass or plasticware or to create RNase-free solutions and water. DEPC inactivates RNases by covalent modification.
  Added: Sat Apr 23 2005, Reviews: 0  Write review  
• Avoiding Ribonucleases Contamination (NEB)
  Why RNA is more susceptible to degradation than DNA and how to avoid it...
  http://www.neb.com/neb/tech/tech_resource/miscella...
  Added: Tue May 14 2002, Hits: 1409, Reviews: 0  Write review   Cached
• Decontamination and Inhibition of Ribonucleases (Brinkmann)
  RNA is more susceptible to degradation than DNA. Unlike Deoxyribonucleases (DNases), which require metal ions for activity (and can therefore easily be inactivated with chelating agents such as EDTA), ribonucleases (RNases) have virtually no cofactor requirements and advantage of the 2’ hydroxyl groups adjacent to the phosphodiester linkages in RNA as a reactive species.
  http://www.brinkmann.com/purif_decontamination.asp
  Added: Tue May 14 2002, Hits: 1117, Reviews: 0  Write review   Cached
• Diethyl pyrocarbonate (DEPC) Treatment of Aqueous Solutions (Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation)
  Enzyme inactivation with Diethyl Pyrocarbonate (DEPC)
  http://omrf.ouhsc.edu/~frank/DEPC.html
  Added: Thu Sep 01 2005, Hits: 1456, Reviews: 0  Write review   Cached
• Linear Polyacrylamide as Carrier in Ethanol Precipitation of Nucleic Acids (Hideaki Shiraishi, Kyoto University)
  Linear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with ethanol. Although glycogen is often used as carrier in ethanol precipitation, it has been shown that glycogen inhibits the activity of an ssDNA-binding protein on which linear polyacrylamide has no effect. Here is the method on how to prepare linear polyacrylamide used as carrier in ethanol precipitation of nucleic acids.
  http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
  Added: Fri Jan 30 2004, Hits: 1841, Reviews: 0  Write review   Cached
• Preparation of solutions and equipment for isolation of RNA (Practical Approach Online, Oxford University Press)
  http://www.oup.co.uk/pdf/pas/5-7-1.pdf
  Added: Sat Aug 17 2002, Hits: 3306, Reviews: 0  Write review  
• RNA Quality Control (FlyChip)
  Any extracted RNA must be devoid of contaminants such as salt, protein, solvents and genomic DNA. This guidance describes how to use gel electrophoresis check for genomic DNA contamination and RNA degradaton, and use spectrophotometry to check for RNA yield and contamination by salt, solvent, protein, etc.
  http://www.flychip.org.uk/protocols/gene_expressio...
  Added: Tue Aug 22 2006, Hits: 1029, Reviews: 0  Write review   Cached
• RNA Quality Control (OpenWetWare)
  This protocol will assist you in performing RNA quality control for a variet of purposes; Microarray, real-time and Northern analysis.
  http://openwetware.org/wiki/RNA_Quality_Control
  Added: Sun Mar 04 2007, Hits: 548, Reviews: 0  Write review   Cached
• RNase Activity Staining on SDS-PAGE (Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
  A detailed procedure for RNase activity staining on SDS-PAGE including recepes.
  http://www.bch.msu.edu/pamgreen/rnase.htm
  Added: Sat Oct 25 2003, Hits: 565, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• Why is isopropanol or ethanol used to precipitate nucleic acids?
• How to precipitate small quantity of RNA?
• In what should RNA be diluted for spectrophotometric RNA quantitation at 260 nm?
• Long term storage of RNA
• Tips on removing DNA contamination from RNA
• Transfer samples from Surgical Pathology to molecular lab
• mRNA quantification- spectrophotometric method
• Does autoclave kill RNases/DNases?
• Tips on determining RNA concentration
• Issues about RNAlater
• Tips on preparing DEPC-treated water and buffers
• Tips on RNA storage
• Does RNA degradate in the refridgerator (4C)?
• How to measure the concentration of RNA?

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