Related Categories:
Protocols
- RNA Quantitation and Purity Determination (Qiagen)
The concentration of RNA should be determined by measuring the absorbance at 260 nm (A260) in a spectrophotometer.
Added: Sat Apr 23 2005, Reviews: 0 Write review -
Quantifying RNA by Spectral Absorption
(Hubbard Center for Genome Studies and Univ. of New Hampshire)
RNA can be quantified using absorption of light at 260 and 280 nm (A260/280). Ideally, this ratio should be close to 2 for high quality nucleic acid
http://hcgs.unh.edu/protocol/basic/rnaabs.html
Added: Sat Apr 23 2005, Hits: 2393, Reviews: 0 Write review Cached