Category: Experimental Procedures
Author: Admin eLabJournal
Labels: Electrophoresis, RNA
|Volume of gel||ml |
|Agarose percentage||% |
formaldehyde-agarose gel of ml
Dissolve in the microwave and allow to cool to 50°C
Perform all next steps in the fumehood
Add of ml
of 37%-formaldehyde and add 1-2 µl of ethidiumbromide
Swirl and pour the agarose-solution into the gel tray, place a comb with the appropriate number of wells and allow the gel to solidify
Remove the comb, place the gel in a gel running system such that the gel is submerged in 1X MOPS buffer
of RNA in µl
of denaturation mix
for 15 min at 55°C.
Mix the denatured RNA samples with µl
of loading buffe
r and load the samples on gel
Run the gel at 40 mA for 45 min and visualize the RNA in a UV-transilluminator.
- Make sure that all used materials are RNase free.
- Use gloves and change them regularly.