Top : Molecular Biology : RNA : Northern Blotting

Summary: Northern blotting or Northern hybridization is a technique for detection of specific RNA sequences. Northern blotting was developed by James Alwine and George Stark at Stanford University and was named such by analogy to Southern blotting. Northern blotting involves the following steps: 1) Total cellular RNA or mRNA is size-separated by denaturing agarose gel electrophoresis, 2) The separated RNA is transferred onto a nylon membrane; 3) The RNA is then detected with isotopic or non-isotopic labeled complementary DNA or RNA probe.
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Protocols
  • Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel (Contributed by Zuyuan Qian)
    This is the standard and complete Northern blotting protocol including preparation of RNA gel , RNA sample, and probe, hybridization, detection and recipes for solutions
    Added: Tue May 14 2002, Reviews: 0  Write review  
  • Glyoxal/DMSO Gel (Carl Clark)
    Glyoxal/DMSO gels are useful for determining sizes of RNA especially in conjunction with Northern blots.
    http://www.cbs.umn.edu/~kclark/protocols/glyoxal.h...
    Added: Tue May 14 2002, Hits: 331, Reviews: 0  Write review   Cached
  • Hybridizing Northern Blots with Riboprobes (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 592, Reviews: 0  Write review   Cached
  • microRNA Northern blot (John Lab - University of pittsburgh)
    See page 7 of the document for detailed step-by-step protocol for microRNA and other small RNA northen blotting
    http://nar.oxfordjournals.org/content/suppl/2010/0...
    Added: Mon Aug 22 2011, Hits: 2588, Reviews: 0  Write review  
  • Northern Blot (Bowtell Lab)
    RNA gel and transfer
    http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
    Added: Tue May 14 2002, Hits: 2460, Reviews: 0  Write review   Cached
  • Northern blot protocol in triethanolamine system (combination of commercial protocols (Pharmacia) with my original modifications)
    This is a simple and reliable method for separation of RNA. It takes the best of few classic protocols including the protocol supplied earlier by "Pharmacia" before this company merged with "Amersham". The protocol takes two days to complete. For your convenience, I included all necessary formulations as well as indicated the steps where you can either break for the lunch or leave your stuff overnight ;) The protocol is good for total RNA samples. I have used this protocol intensively for 5 years
    http://www.geocities.com/mesentsev/Biblioteque/mon...
    Added: Wed Jun 02 2004, Hits: 1573, Reviews: 0  Write review   Cached
  • Northern Blots (Carl Clark)
    Detailed protocol for RNA gel, transfer and hybridization
    http://biosci.cbs.umn.edu/~kclark/protocols/northe...
    Added: Tue May 14 2002, Hits: 4906, Reviews: 0  Write review   Cached
  • Northern Blots ( Michael Koelle) (Ambros Lab)
    http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
    Added: Tue May 14 2002, Hits: 3644, Reviews: 0  Write review   Cached
  • Northern Blotting (Howell Lab)
    Detailed protocol for procedures from RNA isolation, sample preparation, to blot hybridization
    http://cancer.ucsd.edu/howelllab/Northern.html
    Added: Tue May 14 2002, Hits: 6672, Reviews: 0  Write review   Cached
  • Northern Blotting (Breeden Lab)
    RNA electrophoresis, transfer, crosslink and hybridization...
    http://www.fhcrc.org/labs/breeden/Methods/Northern...
    Added: Tue May 14 2002, Hits: 4687, Reviews: 0  Write review   Cached
  • Northern Blotting (Gottschling Lab, Fred Hutchinson Cancer Research Center )
    Describes RNA gel and tranfer.
    http://labs.fhcrc.org/gottschling/General%20Protoc...
    Added: Fri Jul 26 2002, Hits: 3821, Reviews: 0  Write review   Cached
  • Northern Hybridization (Laurie Lab, University of Virginia Health Science Center)
    Detailed Protocol including procedures for preparing poly A+ RNA, running gel and hybridization...
    http://people.virginia.edu/~gwl6s/home.html/Method...
    Added: Tue May 14 2002, Hits: 3209, Reviews: 0  Write review   Cached
  • Northern Transfer Protocol (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 594, Reviews: 0  Write review   Cached
  • Pre-hyb/hyb for Radioactive Probes (Gottschling Lab, Fred Hutchinson Cancer Research Center)
    Norther hybridization with radioacive probe.
    http://www.fhcrc.org/labs/gottschling/misc/rad.htm...
    Added: Fri Jul 26 2002, Hits: 1781, Reviews: 0  Write review   Cached
  • Protocol for Northern Blotting Analysis PDF file (Steven Finkbeiner, UCSF)
    http://www.gladstone.ucsf.edu/gladstone/files/fink...
    Added: Fri Feb 20 2009, Hits: 4684, Reviews: 0  Write review