Top : Molecular Biology : RNA : Northern Blotting

Summary: Northern blotting or Northern hybridization is a technique for detection of specific RNA sequences. Northern blotting was developed by James Alwine and George Stark at Stanford University and was named such by analogy to Southern blotting. Northern blotting involves the following steps: 1) Total cellular RNA or mRNA is size-separated by denaturing agarose gel electrophoresis, 2) The separated RNA is transferred onto a nylon membrane; 3) The RNA is then detected with isotopic or non-isotopic labeled complementary DNA or RNA probe.
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