This is a cached page for the URL (http://biosci.cbs.umn.edu/~kclark/protocols/northern.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Northern Blotting

Northern Blotting

Adapted from Molecular Cloning (Maniatis), Current Protocols in Molecular Biology, and J. Yost Lab (UMN)

Objective:

Northern blotting allows detection of specific RNA sequences. RNA is fractionated by agarose gel electorphoresis, followed by transfer (blotting) to a membrane support, followed by hybridization with DNA or RNA probes.

Procedures:

    Start=> This protocol requires isolated total RNA or poly(A)+ RNA.

  1. You must first run the RNA on a denaturing gel; a formaldehyde or glyoxal/DMSO gel.
  2. Next, you need to transfer the RNA from the gel to a solid support membrane, preferably nylon.
  3. Once, the membrane with the immobilized RNA is available, hybridization with the appropriate probe is required.

Direct questions or comments concerning this web page to Karl Clark: kclark@biosci.cbs.umn.edu
Last modified September 04, 1997
URL = http://biosci.cbs.umn.edu/~kclark/protocols/northern.html%3CBR>
The University of Minnesota is an equal opportunity educator and employer.

© 1996 by the Regents of the University of Minnesota.
All Rights Reserved.