Protocols
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RNA dot blot and slot blot (
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Beverly Faulkner-Jones)
This methods allows the rapid analysis of numerous small samples for the sequence of interest and is less time consuming than the gel electrophoresis methods. 'Dots' or 'slots' of RNA are made onto a filter using a manifold and the filter is then hybridized with a labeled probe. Partially degraded RNA can also be used with good semi-quantitative results. However, high false positive rates need careful use of appropriate controls
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 952, Reviews: 0 Write review Cached -
RNA/Zeta Probe Dot Blotting
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
http://www.nottingham.ac.uk/~mbzspd/methods/RNA-Ze...
Added: Sat Apr 10 2004, Hits: 148, Reviews: 0 Write review Cached