2) Apply a vacuum across the apparatus to draw the solution through the membrane and wash each dot once with 500ul ice-cold 10mM NaOH, 1mM EDTA.
3) Remove membrane from apparatus and wash for 5 mins in 2 x SSC at room temperature.
4) Allow membrane to air dry.
At this stage the membrane can be stored dry at -20°C until required for hybridisation to the probe of choice or it can be used immediately.
Dr. Simon Dawson,
Department of Biochemistry,
University of Nottingham,
The Medical School ,
Clifton Boulevard ,
Tel: +44 (0)115 9249924 ex.44787,
FAX: +44 (0)115 9422225,
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