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Protocols
  • Large Scale M13RF Isolation (Roe Lab)
    After the M13RF containing bacterial cells are harvested by centrifugation, the double-stranded molecules are isolated using the cesium chloride method as for large scale plasmid isolation...
    http://www.genome.ou.edu/protocol_book/protocol_pa...
    Added: Tue May 14 2002, Hits: 404, Reviews: 0  Write review   Cached
  • Large-scale M13 prep: (GSC, WU)
    http://genome.wustl.edu/tools/protocols/production...
    Added: Tue May 14 2002, Hits: 389, Reviews: 0  Write review  
  • M13 Phage (Michael Blaber)
    Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phage culture, it will be great beneficial to read this.
    http://wine1.sb.fsu.edu/bch5425/lect33/lect33.htm
    Added: Tue May 14 2002, Hits: 272, Reviews: 0  Write review   Cached
  • Phage Miniprep ( )
    An improved method for small scale prep. of bacteriophage DNA based on phage precipitation by zinc chloride.
    http://www2.iastate.edu/~ippm/phage.html
    Added: Tue May 14 2002, Hits: 106, Reviews: 0  Write review   Cached
  • Phage Purification with DE52 (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 67, Reviews: 0  Write review   Cached
  • Preparation of Single-stranded DNA (OGMP)
    This protocol is Optimized for preparing ssDNA from phagemid pBluescript propagated in E.coli host. Two method are described: triton Method and SDS Method.
    http://megasun.bch.umontreal.ca/ogmp/ssdna.html
    Added: Tue May 14 2002, Hits: 355, Reviews: 0  Write review   Cached
  • Single Stranded Template from pBluescript Phagemid (NMFS/NOAA)
    http://micro.nwfsc.noaa.gov/protocols/SK_ssDNA.htm...
    Added: Tue May 14 2002, Hits: 194, Reviews: 0  Write review  
  • Single-Stranded M13 DNA Isolation Using Phenol (Roe Lab)
    This isolation procedure is the method of choice for preparation of M13-based templates to be used in dye-terminator sequencing reactions.
    http://www.genome.ou.edu/protocol_book/protocol_pa...
    Added: Tue May 14 2002, Hits: 278, Reviews: 0  Write review   Cached