Protocols
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Large Scale M13RF Isolation
(Roe Lab)
After the M13RF containing bacterial cells are harvested by centrifugation, the double-stranded molecules are isolated using the cesium chloride method as for large scale plasmid isolation...
http://www.genome.ou.edu/protocol_book/protocol_pa...
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Large-scale M13 prep:
(GSC, WU)
http://genome.wustl.edu/tools/protocols/production...
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M13 Phage
(Michael Blaber)
Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phage culture, it will be great beneficial to read this.
http://wine1.sb.fsu.edu/bch5425/lect33/lect33.htm
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Phage Miniprep
( )
An improved method for small scale prep. of bacteriophage DNA based on phage precipitation by zinc chloride.
http://www2.iastate.edu/~ippm/phage.html
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Phage Purification with DE52
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Preparation of Single-stranded DNA
(OGMP)
This protocol is Optimized for preparing ssDNA from phagemid pBluescript propagated in E.coli host. Two method are described: triton Method and SDS Method.
http://megasun.bch.umontreal.ca/ogmp/ssdna.html
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Single Stranded Template from pBluescript Phagemid
(NMFS/NOAA)
http://micro.nwfsc.noaa.gov/protocols/SK_ssDNA.htm...
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Single-Stranded M13 DNA Isolation Using Phenol
(Roe Lab)
This isolation procedure is the method of choice for preparation of M13-based templates to be used in dye-terminator sequencing reactions.
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 443, Reviews: 0 Write review Cached