Protocols
- PCR (Fermentas)
Gives detailed general protocol for PCR, including preparing reaction mixture, cycling conditions, guidelines to avoid contamination and more...
http://www.fermentas.com/techinfo/PCR/dnaamplproto...
Added: Tue May 14 2002, Hits: 41822, Reviews: 1 Read reviews Write review Cached - PCR Manual (Roche)
This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.
https://www.roche-applied-science.com/fst/publicat...
Added: Tue May 14 2002, Hits: 21315, Reviews: 0 Write review Cached -
Calculating Concentrations for PCR
(Molecular Biology Techniques Manual)
How to calculate primer and nucleotide concentration
http://www.mcb.uct.ac.za//pcrconcn.htm
Added: Tue May 14 2002, Hits: 15989, Reviews: 0 Write review Cached -
Detect Alu Repeat by PCR
(Biotech)
PCR is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene.
http://www.accessexcellence.com/AE/AEPC/DNA/detect...
Added: Tue May 14 2002, Hits: 1542, Reviews: 0 Write review Cached -
PCR
(Dr. Chen, Dept of Biochem. & Mol. Biology, Univ. College London)
Standard PCR protocol with detailed notes on every aspects of PCR, such as cycling conditions, components used, additives, and primer design.
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/pro...
Added: Tue May 14 2002, Hits: 21768, Reviews: 0 Write review Cached -
PCR
(Julie B. Wolf, University of Maryland, Baltimore County)
PCR components and cycling conditions
http://userpages.umbc.edu/~jwolf/m2.htm#pcr
Added: Tue May 14 2002, Hits: 8746, Reviews: 0 Write review Cached -
PCR
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 2862, Reviews: 0 Write review Cached -
PCR and multiplex PCR guide
(Octavian Henegariu, Yale University)
Detailed guide for PCR optimization, like concentration of various components, cycling conditions, adjuvants and more.
http://info.med.yale.edu/genetics/ward/tavi/Guide....
Added: Tue May 14 2002, Hits: 15646, Reviews: 0 Write review Cached -
PCR Guide
(Michael Blaber's Lab)
Practical and useful guide to selection of polymerase, primer design, and more...
http://wine1.sb.fsu.edu/bch5425/lect23/lect23.htm
Added: Tue May 14 2002, Hits: 6251, Reviews: 0 Write review Cached -
PCR Guide
(Qiagen)
General guide to primer design, degenerate primer design and long PCR cycling conditions.
http://www1.qiagen.com/resources/info/guidelines_f...
Added: Tue May 14 2002, Hits: 5366, Reviews: 0 Write review Cached