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Protocols
- PCR (Fermentas)
Gives detailed general protocol for PCR, including preparing reaction mixture, cycling conditions, guidelines to avoid contamination and more...
http://www.fermentas.com/techinfo/PCR/dnaamplproto...
Added: Tue May 14 2002, Hits: 17085, Reviews: 0 Write review Cached - PCR Manual (BMB)
This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.
http://206.53.227.20/prod_inf/manuals/pcr_man/star...
Added: Tue May 14 2002, Hits: 2822, Reviews: 0 Write review Cached -
PCR
(Chen)
Standard PCR protocol with detailed notes on every aspects of PCR, such as cycling conditions, components used, additives, and primer design.
http://www.biochem.ucl.ac.uk/~chen/protocols/PCR.h...
Added: Tue May 14 2002, Hits: 21260, Reviews: 0 Write review -
Calculating Concentrations for PCR
(Molecular Biology Techniques Manual)
How to calculate primer and nucleotide concentration
http://web.uct.ac.za/microbiology/pcrconcn.htm
Added: Tue May 14 2002, Hits: 13865, Reviews: 0 Write review Cached -
Detect Alu Repeat by PCR
(Biotech)
PCR is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene.
http://www.accessexcellence.com/AE/AEPC/DNA/detect...
Added: Tue May 14 2002, Hits: 1683, Reviews: 0 Write review Cached -
PCR
(Julie B. Wolf, University of Maryland, Baltimore County)
PCR components and cycling conditions
http://www.research.umbc.edu/~jwolf/m2.htm#pcr
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PCR
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 788, Reviews: 0 Write review Cached -
PCR and multiplex PCR guide
(Octavian Henegariu, Yale University)
Detailed guide for PCR optimization, like concentration of various components, cycling conditions, adjuvants and more.
http://info.med.yale.edu/genetics/ward/tavi/Guide....
Added: Tue May 14 2002, Hits: 19562, Reviews: 0 Write review Cached -
PCR Guide
(Michael Blaber's Lab)
Practical and useful guide to selection of polymerase, primer design, and more...
http://wine1.sb.fsu.edu/bch5425/lect23/lect23.htm
Added: Tue May 14 2002, Hits: 1120, Reviews: 0 Write review Cached -
PCR Guide
(Qiagen)
General guide to primer design, degenerate primer design and long PCR cycling conditions.
http://www.qiagen.com/resources/info/guidelines_fo...
Added: Tue May 14 2002, Hits: 8562, Reviews: 0 Write review Cached -
PCR Guide
(LaboratoryExperiments.com)
Basic guide high school students, But very useful if you are new to molecular biology.
http://www.laboratoryexperiments.com/LAB2.htm
Added: Tue May 14 2002, Hits: 6035, Reviews: 0 Write review Cached -
PCR Program Design
(Octavian Henegariu)
For any given primer pair, the PCR program can be selected based on the composition (GC content) of the primers and the length of the expected PCR product. Here is a guide to select the cycling program of PCR.
http://info.med.yale.edu/genetics/ward/tavi/p08.ht...
Added: Tue May 14 2002, Hits: 4669, Reviews: 0 Write review Cached -
PCR protocol
(eLabProtocols)
http://www.elabprotocols.com/viewer/?id=233
Added: Wed Sep 08 2010, Hits: 3653, Reviews: 0 Write review Cached -
Standard PCR
(Biotech)
Detailed PCR protocol including preparation of the sample, the master mix and the primers, detection and analysis of the reaction products.
http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_t...
Added: Tue May 14 2002, Hits: 9337, Reviews: 0 Write review Cached -
Standard PCR
(Chen)
Standard PCR, primer design, cycling, additives and more
http://www.biochem.ucl.ac.uk/~chen/protocols/PCR.h...
Added: Tue May 14 2002, Hits: 5835, Reviews: 0 Write review -
Standard PCR Protocol
(Molecular Biology Techniques Manual)
The followings are described in detail
Recommended Reagent Concentrations
Recommended Reaction Conditions
"Hot Start" PCR
Asymmetric PCR for ssDNA Production
Detecting Products
Labeling PCR Products with Digoxigenin
Cleaning PCR Products
Sequencing PCR Products
Cloning PCR Products
http://web.uct.ac.za/microbiology/pcrcond.htm#Labe...
Added: Tue May 14 2002, Hits: 12562, Reviews: 1 Read reviews Write review Cached -
What's PCR?
(Michael Blaber's Lab)
Illustrated introduction to various aspects of PCR technique. It's very valuable not only for those new to PCR, but also those familiar with PCR.
http://wine1.sb.fsu.edu/bch5425/lect22/lect22.htm
Added: Tue May 14 2002, Hits: 691, Reviews: 0 Write review Cached
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