Summary: Methylation Specific PCR (MSP) is a bisulfite conversion based PCR technique for the study of DNA CpG methylation. For MSP experiment, two pairs of primers are needed with one pair specific for methylated DNA (M) and the other for unmethylated DNA (U). To achieve discrimination for methylated and unmethylated DNA, in each primer (or at least one of the pair) sequence, one or more CpG sites are included. First, DNA is modified with sodium bisulfite and purified. Then, two PCR reactions are performed using M primer pair and U primer pair. Successful amplification from M pair and U pair indicated methylation and unmethylation respectively.
Methylation-Sensitive PCR (MSP)(Issa Lab) Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the methylation status of CpG islands. This approach allows the determination of methylation patterns from very small samples of DNA, including those obtained from paraffin-embedded samples, and can be used in the study of abnormally methylated CpG islands in neoplasia, in studies of imprinted genes, and in studies of human tumors for clonality by studying genes inactivated on the X chromosome http://www.mdanderson.org/departments/methylation/... Added: Tue May 14 2002, Hits: 5724, Reviews: 0Write reviewCached