Top : Molecular Biology : PCR : Bisulfite Conversion Based PCR : Methylation Specific PCR (MSP)

Summary: Methylation Specific PCR (MSP) is a bisulfite conversion based PCR technique for the study of DNA CpG methylation. For MSP experiment, two pairs of primers are needed with one pair specific for methylated DNA (M) and the other for unmethylated DNA (U). To achieve discrimination for methylated and unmethylated DNA, in each primer (or at least one of the pair) sequence, one or more CpG sites are included. First, DNA is modified with sodium bisulfite and purified. Then, two PCR reactions are performed using M primer pair and U primer pair. Successful amplification from M pair and U pair indicated methylation and unmethylation respectively.
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