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  • Bisulfite Modification (Conversion) of DNA (Protocol Online)
    Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detecting methylated cytosines using methylation specific PCR (MSP) technique or bisulfite genomic sequencing after PCR amplification with or without cloning. This protocol also describes procedure for handling nanogram quantities of DNA.
    Added: Fri May 07 2004, Reviews: 0  Write review  
  • Plant DNA Methylation Analysis by Bisulfite Genomic Ssequencing (The Epigenome Network of Excellence )
    This protocol is optimized for the determination of DNA cytosine methylation at transgene loci in plants.
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Sat Mar 18 2006, Hits: 1424, Reviews: 0  Write review   Cached
  • Analysis of Methylation using Bisulphite Sequencing (Methods.info)
    This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated cytosines remain unchanged.
    http://www.methods.info/Methods/DNA_methylation/Bi...
    Added: Tue Aug 10 2004, Hits: 5610, Reviews: 0  Write review   Cached
  • Bisulfite Genomic DNA Sequencing PDF file (Craig Pikaard's Laboratory at Washington University)
    http://biology4.wustl.edu/pikaard/PDFs%20and%20pro...
    Added: Sun Mar 02 2008, Hits: 580, Reviews: 0  Write review  
  • Bisulfite Genomic Sequencing (Methods.info)
    This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated cytosines remain unchanged. This method requires small amount of genomic DNA and therefore seems to be very useful for the analysis of clinical samples, where the material amount is limited.
    http://www.methods.info/Methods/DNA_methylation/Bi...
    Added: Sat Dec 20 2003, Hits: 4706, Reviews: 0  Write review   Cached
  • Bisulfite modification of DNA (Fan Lab, UCLA)
    This is a well-written protocol for bisulfite treatment of DNA with something unique. DNA is first digested with a restriction enzyme and then denatured at 97C for 5min. After bisulfite treatment, DNA is purified using Promega Wizard DNA Cleanup kit.
    http://www.genetics.ucla.edu/labs/fan/Protocols_Bi...
    Added: Sat Jan 15 2005, Hits: 3887, Reviews: 0  Write review   Cached
  • Bisulfite Sequencing of Small DNA/Cell Samples (Epigenome Network of Excellence)
    Here we describe a protocol for small numbers of cells and little DNA which requires some specific handling. The protocol is based on a strategy originally introduced by our lab (Hajkova et al., 2002) using agarose embedded DNA. This physical trapping helps to avoid DNA loss during the various incubation steps while maintaining a good bisulphite conversion rate. We will introduce two alternative procedures to perform bisulphite treatment of agarose embedded small DNA aliquots or cells and guide through some generally critical points in the bisulphite reaction and primer design.
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Mon Aug 04 2008, Hits: 74, Reviews: 0  Write review  
  • Bisulfite Sequencing of Very small Samples (Epigenome Network of Excellence)
    This protocol is optimized for bisulfite sequencing of small samples where the starting material was a small number of cells. The smallest amount of material from which several unique clones were recovered was 25 oocytes, which corresponds to 100 DNA molecules in the initial material. This protocol can be also used for analyzing up to 200ng purified genomic DNA in one sample
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Mon Aug 04 2008, Hits: 51, Reviews: 0  Write review  
  • Bisulfite Treatment of DNA (Issa Lab)
    DNA treatment for methylation mapping PCRs such as MSP and bisulfite sequencing PCR
    http://www.mdanderson.org/departments/methylation/...
    Added: Tue May 14 2002, Hits: 4873, Reviews: 0  Write review   Cached
  • Plant DNA methylation analysis by "bisulfite genomic sequencing" (Epigenome NoE)
    The "bisulfite genomic sequencing" protocol has been optimized for the determination of DNA cytosine methylation at transgene loci in plants. The conditions used are based on the method given by Pelissier and Wassenegger (2000).
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Mon Aug 04 2008, Hits: 25, Reviews: 0  Write review  
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