- Making T-A cloning Vectors (Chin-Sang Lab, Queen's University, Kingston, ON, Canada)
A method for direct cloning a PCR product, by the T-vector technique. This is cheap and easy way to clone PCR products with A 3’ overhangs.
Added: Fri Feb 06 2009, Hits: 3070, Reviews: 0
Construction of Homemade 'T-vectors'
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Making T vector from plasmid for TA cloning
Added: Sat Apr 10 2004, Hits: 234, Reviews: 0
How to make a T/A vector
Added: Fri Feb 06 2009, Hits: 1709, Reviews: 0
(Delaney Lab at the University of Vermont)
This protocol should work on the basis of the fact that given an equimolar amount of dNTPs in a PCR reaction, Taq DNA polymerase I will add an extra (template-independent) A. If the enzyme is supplied with only dTTP, then an extra T will be added.
Added: Fri Feb 06 2009, Hits: 1652, Reviews: 0