Abstract for Packagene® Lambda DNA Packaging System
The Packagene® Lambda DNA Packaging System is derived from the unique one-strain host system (Rosenberg). The System is easy to use because it contains all the components and buffers needed to perform the packaging reaction in a single tube. Only the addition of substrate DNA is required. The packaging efficiencies of recombinant DNA using the single-strain Packagene® System are as efficient as those using a two-strain system.
The Packagene® Extract is produced from an E. coli C bacterial host. Libraries constructed using the Packagene® System are not biased by EcoK restriction of genetic material because E. coli C lacks the K-12 DNA restriction system. The Positive Control DNA provided with the system, lambda cI857 Sam7, allows evaluation of Packagene® System performance without depleting sample DNA.