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manual of BAC Protocols in hbz lab (plant genomics)
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Manual of BAC Protocols


Introduction Construction of Large-Insert Bacterial Clone Libraries
Experiment  1   Preparation of High-quality Megabase-size DNA
Preparation of Megabase-size Nuclear DNA from Plants
1.2 Preparation of Megabase-size DNA from Animals
1.3 Preparation of Megabase-size DNA from Insects
Experiment  2 Preparation of BAC and BIBAC Cloning Vectors
Experiment  3

Generation of Large DNA Fragments from Megabase DNA Embedded in LMP Agarose by Partial Digestion for BAC and BIBAC Cloing: Determination of Optimal Partial Digestion Conditions

Experiment  4 BAC and BIBAC Cloning: Partial Digestion of Megabase DNA, Size Selection, Ligation and Transformation 
Experiment  5 BAC Analysis
Experiment  6 Large-insert, Ordered Bacterial Clone Library Manipulation
Experiment  7 BAC and BIBAC Library Screening
Experiment  8 BAC End Isolation
Appendix  A Preparation of Yeast Chromosome-size DNA and Lambda DNA Ladder
Appendix  B Preparation of Competent Cells for Bacterial Transformation by Electroporation
Appendix  C Chemicals and Supplies
Appendix  D Solutions and Preparation
Appendix  E Restriction Site Maps of Widely Used BAC, BIBAC, PBC and PAC Vectors
Appendix  F Nucleotide Sequences:  pBeloBAC11, pCLD04541 and pBACe3.6
References Cited  

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manual of BAC protocols.

Last Updated Thursday October 03, 2002 Questions ?  
Copyright 2002 hbz laboratory all rights reserved.
Norman E. Borlaug Center, College Station, TX77843