Protocols

• Preparation of blunt-end DNA fragments (Practical Approach Online, Oxford University Press)
  Two methods are introduced: Filling in 5'-overhangs with Klenow DNA polymerase and removal of 3'-overhangs by the exonuclease reaction of T4 DNA polymerase
  http://www.oup.co.uk/pdf/pas/9v1-7-10.pdf
  Added: Sat Aug 17 2002, Hits: 2312, Reviews: 0  Write review  
• Applications of Modifying Enzymes (NEB)
  Presents quick reference table for application of modifying enzymes.
  http://www.neb.com/neb/tech/tech_resource/modifyin...
  Added: Tue May 14 2002, Hits: 336, Reviews: 0  Write review   Cached
• Electroelution of DNA Fragments From Agarose into Dialysis Tubing (Donis Keller Lab)
  To retrieve and purify any specific DNA fragment from an agarose gel slice; the expected yield is from 50-75% of the amount in the gel slice.
  http://humgen.wustl.edu/hdk_lab_manual/rdm/rdm9.ht...
  Added: Tue May 14 2002, Hits: 234, Reviews: 0  Write review   Cached
• Fill-In Reaction (Hedrick Lab, UCSD Cancer Center)
  Reaction for generating blunt ends on dsDNA fragments with overhangs (especially good for 5' overhangs).
  http://hedricklab.ucsd.edu/Protocol/FillIn.html
  Added: Tue Dec 10 2002, Hits: 1222, Reviews: 0  Write review   Cached
• Thermal Inactivation (Fermentas)
  A simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If further manipulations of the digested DNA are to be performed, the restriction endonuclease should be inactivated. Phenol/chloroform extraction and ethanol precipitation is an irreversible method for inactivation and removal of all restriction endonucleases...
  http://www.fermentas.com/techinfo/RE/restrthermina...
  Added: Tue May 14 2002, Hits: 294, Reviews: 0  Write review   Cached