| Bst DNA L.Frag | Taq | Vent | Vent (exo-) | Deep Vent | Deep Vent (exo-) | 9°Nm | Ther- minator | T7 DNA | E.coli Poly I | Klenow Frag | Klenow exo- | Reverse Transc- riptase | T4 DNA. | T7 RNA | SP6 RNA | Labeling in vitro | 3' fill-in | (+) | + | (+) | + | (+) | + | (+) | | (+) | (+) | (+) | + | | (+) | | | 3' exchange | | | + | | + | | | | + | (+) | + | | | + | | | nick-translation | | + | | | | | | | | + | | | | | | | replacement synthesis | | | (+) | | (+) | | | | (+) | (+) | (+) | | | + | | | random priming | + | + | + | + | + | + | + | | | | + | + | | | | | RNA probes | | | | | | | | | | | | | | | + | + | Cloning | creating blunt ends | 5' overhang fill-in | | | (+) | | (+) | | (+) | | (+) | (+) | + | | | + | | | 3' overhang removal | | | + | | + | | (+) | | (+) | (+) | + | | | + | | | nested deletions | | | | | | | | | | | | | | + | | | Sequencing (DNA) | DNA | (+) | (+) | | (+) | | (+) | | + | | (+) | + | (+) | (+) | | | | RNA | | | | | | | | | | | | | + | | | | Mutagenesis | oligonucleotide directed | | | (+) | | (+) | | | | + | | (+) | | | + | | | misincorporation | | + | | | | | | | | | + | | | | | | Mapping | transcript | | | | | | | | | | | | | + | | | | cDNA | synthesis | 1st strand | | | | | | | | | | | | | + | | | | 2nd strand | (+) | | (+) | | (+) | | | | | (+) | + | | | | | | cloning | Okayama-Berg | | | | | | | | | | | | | + | | | | In vitro transcription | | | | | | | | | | | | | | | + | + | + = recommended enzyme for this application (+) = usable, but not the enzyme of choice |