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Protocols

• DNA Ligation (Practical Approach Online, Oxford University Press)
  Ligation reaction using T4 ligase.
  http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf
  Added: Sat Aug 17 2002, Hits: 5372, Reviews: 0  Write review  
• Addition of linkers/adaptors to blunt-end DNA fragments (Practical Approach Online, Oxford University Press)
  Very nice protocol with links to related procedures.
  http://www.oup.co.uk/pdf/pas/9v1-7-11.pdf
  Added: Sat Aug 17 2002, Hits: 910, Reviews: 0  Write review  
• DNA Insert Ligation into Vector DNA (with T4 DNA Ligase) (Fermentas)
  http://www.fermentas.com/techinfo/ModifyingEnzymes...
  Added: Tue May 14 2002, Hits: 2108, Reviews: 0  Write review   Cached
• DNA Ligation (Practical Approach Online, Oxford University Press)
  Ligation of DNA fragments by homopolymer tailing
  http://www.oup.co.uk/pdf/pas/9v1-7-13.pdf
  Added: Sat Aug 17 2002, Hits: 2329, Reviews: 0  Write review  
• DNA ligation (Roe, OU)
  DNA ligations are performed by incubating DNA fragments with appropriately linear zed cloning vector in the presence of buffer, rATP, and T4 DNA ligase.
  http://www.genome.ou.edu/protocol_book/protocol_pa...
  Added: Tue May 14 2002, Hits: 2051, Reviews: 0  Write review   Cached
• DNA Ligation (Chen)
  Protocol and useful notes on cohesive end ligation
  http://www.biochem.ucl.ac.uk/~chen/protocols/ligat...
  Added: Tue May 14 2002, Hits: 3131, Reviews: 0  Write review  
• Linker Ligation (with T4 DNA Ligase) (Fermentas)
  http://www.fermentas.com/techinfo/ModifyingEnzymes...
  Added: Tue May 14 2002, Hits: 860, Reviews: 0  Write review   Cached
• Removal of excess linkers/adapters (Practical Approach Online, Oxford University Press)
  Use size-based separation matrix resin column
  http://www.oup.co.uk/pdf/pas/9v1-7-12.pdf
  Added: Sat Aug 17 2002, Hits: 359, Reviews: 0  Write review  
• Self-Circularization of Linear DNA (with T4 DNA Ligase) (Fermentas)
  http://www.fermentas.com/techinfo/ModifyingEnzymes...
  Added: Tue May 14 2002, Hits: 436, Reviews: 0  Write review   Cached
• Standard Ligation (Schneitz Lab)
  Detailed guide for ligation reaction
  http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
  Added: Tue May 14 2002, Hits: 2816, Reviews: 0  Write review   Cached