Protocols
- DNA Ligation
(Practical Approach Online, Oxford University Press)
Ligation reaction using T4 ligase.
http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf
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Addition of linkers/adaptors to blunt-end DNA fragments
(Practical Approach Online, Oxford University Press)
Very nice protocol with links to related procedures.
http://www.oup.co.uk/pdf/pas/9v1-7-11.pdf
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DNA Insert Ligation into Vector DNA (with T4 DNA Ligase)
(Fermentas)
http://www.fermentas.com/techinfo/ModifyingEnzymes...
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DNA Ligation
(Practical Approach Online, Oxford University Press)
Ligation of DNA fragments by homopolymer tailing
http://www.oup.co.uk/pdf/pas/9v1-7-13.pdf
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DNA ligation
(Roe, OU)
DNA ligations are performed by incubating DNA fragments with appropriately linear zed cloning vector in the presence of buffer, rATP, and T4 DNA ligase.
http://www.genome.ou.edu/protocol_book/protocol_pa...
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DNA Ligation
(Chen)
Protocol and useful notes on cohesive end ligation
http://www.biochem.ucl.ac.uk/~chen/protocols/ligat...
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Linker Ligation (with T4 DNA Ligase)
(Fermentas)
http://www.fermentas.com/techinfo/ModifyingEnzymes...
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Removal of excess linkers/adapters
(Practical Approach Online, Oxford University Press)
Use size-based separation matrix resin column
http://www.oup.co.uk/pdf/pas/9v1-7-12.pdf
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Self-Circularization of Linear DNA (with T4 DNA Ligase)
(Fermentas)
http://www.fermentas.com/techinfo/ModifyingEnzymes...
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Standard Ligation
(Schneitz Lab)
Detailed guide for ligation reaction
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 3553, Reviews: 0 Write review Cached