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T4 DNA Ligase 5u. Vortex the tube and spin down in a microcentrifuge for 3-5 seconds.
Incubate the mixture for 1 hour at 22°C .
Inactivate T4 DNA Ligase by heating reaction mixture at 65°C for 10 minutes.
Resulting reaction mixture can be used directly for transformation.
References
Sambrook, J., Russell, D.W., Molecular Cloning: A Laboratory Manual, the third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001.
Current Protocols in Molecular Biology, vol. 1 (Ausubel, F.M., et al., ed.), John Wiley & Sons, Inc., Brooklyn, New York, 1994-2001.