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Protocols

• BAC&PAC Mini Prep (BACPAC Resource Center at the Children's Hospital Oakland Research Institute )
  This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary.
  http://bacpac.chori.org/bacpacmini.htm
  Added: Tue Jul 30 2002, Hits: 398, Reviews: 0  Write review   Cached
• Cycle Sequence Reactions For Large Insert Plasmid Templates (BACPAC Resource Center at the Children's Hospital Oakland Research Institute )
  The dye-labeled terminator reaction chemistries have been designed to balance conservation of reagents with the resulting sequence product signal strength.
  http://bacpac.chori.org/cyclesere.htm
  Added: Tue Jul 30 2002, Hits: 206, Reviews: 0  Write review   Cached
• Preparation of BAC DNA Clones (BACPAC Resource Center at the Children's Hospital Oakland Research Institute)
  Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids
  http://bacpac.chori.org/dnaprep.htm
  Added: Tue Jul 30 2002, Hits: 308, Reviews: 0  Write review   Cached