Protocols
- Yeast Transformation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
Includes high efficiency LiAc transformation, 'Lazy Bones' transformation (plasmid transformations) and electroporation in great detail.
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 14248, Reviews: 0 Write review Cached -
"Lazy Bones" Plasmid Transformation
(Henrik G. Dohlman, The University of North Carolina Chapel Hill)
A quick method for plasmid transformation into yeast.
http://www.med.unc.edu/~hdohlman/lazybones.html
Added: Tue May 14 2002, Hits: 2931, Reviews: 0 Write review Cached -
Competent Yeast Preparation
(Gietz Lab)
This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing
http://www.umanitoba.ca/faculties/medicine/biochem...
Added: Tue May 14 2002, Hits: 1881, Reviews: 0 Write review Cached -
Direct PCR from Whole Yeast Cells
(Namjin Chung)
Zymolyase Method for screening yeast transformants
http://www.musc.edu/bcmb/ceramide/protocols/0003.h...
Added: Tue May 14 2002, Hits: 1106, Reviews: 1 Read reviews Write review Cached -
Fission Yeast Transformation
(Forsburg Lab)
Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion.
http://pingu.salk.edu/~forsburg/tfmn.html
Added: Tue May 14 2002, Hits: 1207, Reviews: 0 Write review Cached -
Large-Scale Yeast Transformation
(Yale Yeast Analysis Center)
Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...
http://ygac.med.yale.edu/mtn/LS_transf_prot.stm
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Modified Yeast Transformation
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Multiwell Transformation
(Davis Lab)
Detailed protocol with LiAcetate PEG transformation method
http://sequence-www.stanford.edu/group/yeast_delet...
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Quick Yeast Transformation
(Gietz Lab)
This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.
http://www.umanitoba.ca/faculties/medicine/biochem...
Added: Tue May 14 2002, Hits: 1194, Reviews: 0 Write review Cached -
Sleazy Plate Transformation of Yeast
(Herskowitz Lab)
http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
Added: Tue May 14 2002, Hits: 624, Reviews: 0 Write review -
Transformation of Yeast
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Transformation of Yeast - LiAc Method
(Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/met8.h...
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Two Hybrid System Transformation
(Gietz Lab)
This Protocol is for 2 Hybrid system screens
http://www.umanitoba.ca/faculties/medicine/biochem...
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Yeast Small Scale Transformation
(Stephens Lab, University of Bristol)
http://www.bch.bris.ac.uk/staff/stephens/trafo.htm
Added: Thu May 27 2004, Hits: 64, Reviews: 0 Write review Cached -
Yeast Transformation
(Gietz Lab)
LiAc/SS-DNA/PEG Transformation
http://www.umanitoba.ca/faculties/medicine/biochem...
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Yeast Transformation
(Breeden Lab)
LiAc method
http://www.fhcrc.org/labs/breeden/Methods/yeast-tr...
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Yeast Transformation
(Herskowitz Lab)
http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
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Yeast Transformation
(Fangman/Brewer lab)
Yeast transformation protocol Using Frozen Competent Cells and Single-stranded DNA as a Carrier
http://fangman-brewer.genetics.washington.edu/tran...
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Yeast Transformation
(D. Amberg)
Electroporation method
http://www.upstate.edu/biochem/amberg/protocols/tr...
Added: Tue May 14 2002, Hits: 734, Reviews: 0 Write review Cached -
Yeast Transformation
(Finley Lab, Wayne State University, School of Medicine)
LiOAc method
http://proteome.wayne.edu/transform.html
Added: Tue May 14 2002, Hits: 1077, Reviews: 0 Write review Cached -
Yeast Transformation (Rapid method)
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
LiOAc method
http://labs.fhcrc.org/hahn/Methods/genetic_meth/ye...
Added: Tue May 14 2002, Hits: 1040, Reviews: 0 Write review Cached -
Yeast Transformation with DMSO/LioAc (high efficiency)
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/genetic_meth/dm...
Added: Tue May 14 2002, Hits: 1286, Reviews: 0 Write review Cached