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Protocols

• Yeast Transformation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  Includes high efficiency LiAc transformation, 'Lazy Bones' transformation (plasmid transformations) and electroporation in great detail.
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 10660, Reviews: 0  Write review   Cached
• "Lazy Bones" Plasmid Transformation (Henrik G. Dohlman, The University of North Carolina Chapel Hill)
  A quick method for plasmid transformation into yeast.
  http://www.med.unc.edu/~hdohlman/lazybones.html
  Added: Tue May 14 2002, Hits: 2214, Reviews: 0  Write review   Cached
• Competent Yeast Preparation (Gietz Lab)
  This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing
  http://www.umanitoba.ca/faculties/medicine/biochem...
  Added: Tue May 14 2002, Hits: 1860, Reviews: 0  Write review   Cached
• Direct PCR from Whole Yeast Cells (Namjin Chung)
  Zymolyase Method for screening yeast transformants
  http://www.musc.edu/bcmb/ceramide/protocols/0003.h...
  Added: Tue May 14 2002, Hits: 863, Reviews: 1 Read reviews  Write review   Cached
• Fission Yeast Transformation (Forsburg Lab)
  Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion.
  http://pingu.salk.edu/~forsburg/tfmn.html
  Added: Tue May 14 2002, Hits: 950, Reviews: 0  Write review   Cached
• Large-Scale Yeast Transformation (Yale Yeast Analysis Center)
  Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...
  http://ygac.med.yale.edu/mtn/LS_transf_prot.stm
  Added: Tue May 14 2002, Hits: 987, Reviews: 0  Write review   Cached
• Modified Yeast Transformation (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 203, Reviews: 0  Write review   Cached
• Multiwell Transformation  (Davis Lab)
  Detailed protocol with LiAcetate PEG transformation method
  http://sequence-www.stanford.edu/group/yeast_delet...
  Added: Tue May 14 2002, Hits: 496, Reviews: 0  Write review   Cached
• Quick Yeast Transformation (Gietz Lab)
  This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.
  http://www.umanitoba.ca/faculties/medicine/biochem...
  Added: Tue May 14 2002, Hits: 1187, Reviews: 0  Write review   Cached
• Sleazy Plate Transformation of Yeast (Herskowitz Lab)
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
  Added: Tue May 14 2002, Hits: 476, Reviews: 0  Write review  
• Transformation of Yeast (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 199, Reviews: 0  Write review   Cached
• Transformation of Yeast - LiAc Method (Lazo Lab)
  http://wheat.pw.usda.gov/~lazo/methods/lazo/met8.h...
  Added: Tue May 14 2002, Hits: 1015, Reviews: 0  Write review   Cached
• Two Hybrid System Transformation (Gietz Lab)
  This Protocol is for 2 Hybrid system screens
  http://www.umanitoba.ca/faculties/medicine/biochem...
  Added: Tue May 14 2002, Hits: 448, Reviews: 0  Write review   Cached
• Yeast Small Scale Transformation (Stephens Lab, University of Bristol)
  http://www.bch.bris.ac.uk/staff/stephens/trafo.htm
  Added: Thu May 27 2004, Hits: 64, Reviews: 0  Write review   Cached
• Yeast Transformation (Gietz Lab)
  LiAc/SS-DNA/PEG Transformation
  http://www.umanitoba.ca/faculties/medicine/biochem...
  Added: Tue May 14 2002, Hits: 853, Reviews: 0  Write review   Cached
• Yeast Transformation (Breeden Lab)
  LiAc method
  http://www.fhcrc.org/labs/breeden/Methods/yeast-tr...
  Added: Tue May 14 2002, Hits: 965, Reviews: 0  Write review   Cached
• Yeast Transformation (Herskowitz Lab)
  http://www.sacs.ucsf.edu/home/HerskowitzLab/protoc...
  Added: Tue May 14 2002, Hits: 680, Reviews: 0  Write review  
• Yeast Transformation (Fangman/Brewer lab)
  Yeast transformation protocol Using Frozen Competent Cells and Single-stranded DNA as a Carrier
  http://fangman-brewer.genetics.washington.edu/tran...
  Added: Tue May 14 2002, Hits: 785, Reviews: 0  Write review   Cached
• Yeast Transformation (D. Amberg)
  Electroporation method
  http://www.upstate.edu/biochem/amberg/protocols/tr...
  Added: Tue May 14 2002, Hits: 716, Reviews: 0  Write review   Cached
• Yeast Transformation (Finley Lab, Wayne State University, School of Medicine)
  LiOAc method
  http://proteome.wayne.edu/transform.html
  Added: Tue May 14 2002, Hits: 823, Reviews: 0  Write review   Cached
• Yeast Transformation (Rapid method) (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  LiOAc method
  http://labs.fhcrc.org/hahn/Methods/genetic_meth/ye...
  Added: Tue May 14 2002, Hits: 826, Reviews: 0  Write review   Cached
• Yeast Transformation with DMSO/LioAc (high efficiency) (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  
  http://labs.fhcrc.org/hahn/Methods/genetic_meth/dm...
  Added: Tue May 14 2002, Hits: 1026, Reviews: 0  Write review   Cached