Protocols
- Yeast Transformation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
Includes high efficiency LiAc transformation, 'Lazy Bones' transformation (plasmid transformations) and electroporation in great detail.
http://www.fhcrc.org/science/labs/gottschling/yeas...
Added: Fri Jul 26 2002, Hits: 11678, Reviews: 0 Write review Cached -
"Lazy Bones" Plasmid Transformation
(Henrik G. Dohlman, The University of North Carolina Chapel Hill)
A quick method for plasmid transformation into yeast.
http://www.med.unc.edu/~hdohlman/lazybones.html
Added: Tue May 14 2002, Hits: 2684, Reviews: 0 Write review Cached -
Competent Yeast Preparation
(Gietz Lab)
This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing
http://www.umanitoba.ca/faculties/medicine/biochem...
Added: Tue May 14 2002, Hits: 3388, Reviews: 0 Write review Cached -
Direct PCR from Whole Yeast Cells
(Namjin Chung)
Zymolyase Method for screening yeast transformants
http://www.musc.edu/bcmb/ceramide/protocols/0003.h...
Added: Tue May 14 2002, Hits: 1250, Reviews: 1 Read reviews Write review Cached -
Fission Yeast Transformation
(Forsburg Lab)
Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion.
http://pingu.salk.edu/~forsburg/tfmn.html
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Large-Scale Yeast Transformation
(Yale Yeast Analysis Center)
Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...
http://ygac.med.yale.edu/mtn/LS_transf_prot.stm
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Lithium Acetate Transformation of Yeast
(Dunham Lab, Lewis-Sigler Institute for Integrative Genomics, Princeton University)
http://www.genomics.princeton.edu/dunham/yeasttran...
Added: Fri Jul 07 2006, Hits: 1486, Reviews: 0 Write review Cached -
Modified Yeast Transformation
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Multiwell Transformation
(Davis Lab)
Detailed protocol with LiAcetate PEG transformation method
http://sequence-www.stanford.edu/group/yeast_delet...
Added: Tue May 14 2002, Hits: 909, Reviews: 0 Write review Cached -
Quick Yeast Transformation
(Gietz Lab)
This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.
http://www.umanitoba.ca/faculties/medicine/biochem...
Added: Tue May 14 2002, Hits: 2391, Reviews: 0 Write review Cached