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Breeden Lab, yeast transformation

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Yeast Transformation




Buffers:

 10 x TE: 100 mM Tris pH 7.5; 10 mM EDTA.
 10 x LiAc: 1 M LiAc in H2O; sterile filter.
 50 % PEG 3350: 50 % PEG 3350 in H2O; sterile filter.