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Protocols

• Confirmation PCR (Saccharomyces Genome Deletion Project)
  To study the genetics of yeast, yeast gene ORF is deleted by PCR based strategy to generate mutated strain. Confirmation PCR is used to verify if the yeast ORF deletion is successful Introduction/General strategy
  Confirmation PCR primer design
  Primer organization in the 96-well plates
  Oligonucleotide concentrations
  Quality control procedures
  Single tube PCR protocol
  96-well format protocol
  http://sequence-www.stanford.edu/group/yeast_delet...
  Added: Tue May 14 2002, Hits: 912, Reviews: 0  Write review   Cached
• Gene Disruption via PCR (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 448, Reviews: 0  Write review   Cached
• Yeast Colonly PCR (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 728, Reviews: 0  Write review   Cached