Protocols
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Confirmation PCR
(Saccharomyces Genome Deletion Project)
To study the genetics of yeast, yeast gene ORF is deleted by PCR based strategy to generate mutated strain. Confirmation PCR is used to verify if the yeast ORF deletion is successful Introduction/General strategy
Confirmation PCR primer design
Primer organization in the 96-well plates
Oligonucleotide concentrations
Quality control procedures
Single tube PCR protocol
96-well format protocol
http://sequence-www.stanford.edu/group/yeast_delet...
Added: Tue May 14 2002, Hits: 1138, Reviews: 0 Write review Cached -
Gene Disruption via PCR
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 583, Reviews: 0 Write review Cached -
Yeast Colonly PCR
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 868, Reviews: 0 Write review Cached