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YeastDeletionWebPages

Databases and Datasets

FAQs

Deletion Strains Available

References

Protocols &Technical Information

Useful Sites & Links

Consortium Members' Addresses

Yeast Deletion Database (consortium members only)

 

Protocols and Technical Information

Technical Information

Yeast Deletion -Overview

Yeast ORF Deletion Strategy
    Cassette Module
    Mutant Construction
    Strain Confimation
     Cell Morphology

Transformation

Gietz Transformation Protocols
Multiwell transformation protocol
ADE2 Transformation control

PCR Protocols

Oligonucleotide details
Single tube PCR protocol
96-well format protocol

Useful Protocols

Sporulation Protocols
Strain Verification Criteria
Pinning from 96-well plates
YPD-filled microtiter plates
Cell Morphology Screen

Maps & Sequences

KanMX4 Sequence and Map
kanB and kanC primer sequences
Primer Sequences