Subcategories

Protocols

• Basic immunofluorescence (Aroian Lab)
  http://www-biology.ucsd.edu/labs/aroian/protocols/...
  Added: Tue May 14 2002, Hits: 1322, Reviews: 0  Write review   Cached
• C. elegans gene knockout protocol (Michael Koelle's Lab)
  The gene knockout strategy involves using a random mutagen (trimethylpsoralen) to mutagenize a very large number of worms. The worms are divided into many small subcultures and allowed to have progeny. A portion of each subculture is stored alive in the freezer, and genomic DNA is made from the rest of the culture.
  http://info.med.yale.edu/mbb/koelle/protocols/prot...
  Added: Tue May 14 2002, Hits: 1147, Reviews: 0  Write review   Cached
• Cleaning Worm Stocks (Michael Koelle) (Ambros Lab)
  The same protocol as above at another site.
  http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
  Added: Tue May 14 2002, Hits: 865, Reviews: 0  Write review   Cached
• Cleaning worm strains (Michael Koelle's Lab)
  Clean fungi and bacteria contaminations.
  http://info.med.yale.edu/mbb/koelle/protocols/prot...
  Added: Tue May 14 2002, Hits: 867, Reviews: 0  Write review   Cached
• Culturing Worms (Michael Koelle's Lab)
  Grow worms and prepare worm food.
  http://info.med.yale.edu/mbb/koelle/protocols/prot...
  Added: Tue May 14 2002, Hits: 1904, Reviews: 0  Write review   Cached
• Dauer Pheromone Preparation: ( )
  MGH
  http://www.dartmouth.edu/artsci/bio/ambros/protoco...
  Added: Tue May 14 2002, Hits: 304, Reviews: 0  Write review   Cached
• Development of techniques for primary culture of ltIgtC. eleganslt/Igt embryonic neurons (Laird Bloo (Ambros Lab)
  Although the techniques for cell culture developed in this section are not fully reproducible, the observation that axons can be grown from cultured C. elegans neurons is encouraging and suggests that further optimization should be attempted. The experiments described here were designed to extend these techniques to the growth of large numbers of dissociated embryonic cells. The goal was to define media, substrates, and cell isolation techniques that would permit neuronal differentiation and axonal outgrowth in short-term cultures...
  http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
  Added: Tue May 14 2002, Hits: 1239, Reviews: 0  Write review   Cached
• dsRNA preparation for RNAi by injection in C. elegans (Goldstein lab)
  This protocol streamlines dsRNA preparation for RNAi by injection in C. elegans
  http://www.bio.unc.edu/faculty/goldstein/lab/dsRNA...
  Added: Mon Sep 20 2004, Hits: 914, Reviews: 0  Write review  
• Egg laying in response to food (Michael Koelle's Lab)
  This method is used to determine how well worms regulate egg laying in response to food quantitatively.
  http://info.med.yale.edu/mbb/koelle/protocols/prot...
  Added: Tue May 14 2002, Hits: 706, Reviews: 0  Write review   Cached
• Embryo Buffers (Aroian Lab)
  http://www-biology.ucsd.edu/labs/aroian/protocols/...
  Added: Tue May 14 2002, Hits: 621, Reviews: 0  Write review   Cached

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