This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache



Immunofluorescence of Embryos


disposable scapel

#15 (round tip) Feather brand

subbed slides

0.1 % poly-L-lysine (MW>300,000)

0.2 % gelatin

0.01 % chrome alum

(dip slides in solution and dry overnight)

Whatmann paper

for ease cut into 1 inch square pieces


  1. Dissect embryos from gravid hermaphrodites
    • pick gravid hermaphrodites into 100 ul of H20 on a subbed slide
    • cut hermaphrodites near vulva with disposable scapel to release embryos
  2. Freeze-fracture embryos
    • remove practically all of liquid from slide with pipet (~90 ul)
    • place coverslip on embryos/worm carcasses
    • wick residual liquid out with Whatmann paper until embryos begin to flatten
    • freeze slide on dry ice >20 minutes
    • quickly flip off coverslip with razor blade
    • immerse slide in fixative
    • process for immunofluorescence



compiled by Chad Rappleye

Aroian Lab Protocols