Top : Model Organisms : C. Elegans
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Protocols
  • Basic immunofluorescence (Aroian Lab)
    http://www-biology.ucsd.edu/labs/aroian/protocols/...
    Added: Tue May 14 2002, Hits: 256, Reviews: 0  Write review   Cached
  • C. Elegans Embryo Staining (Dr. Ian Chin-Sang)
    Staining protocol for C. Elegans embryos for immunofluorescence microscopy.
    http://lab.methodmint.com/methods/1422/
    Added: Mon Feb 06 2012, Hits: 22, Reviews: 0  Write review  
  • C. elegans gene knockout protocol (Michael Koelle's Lab)
    The gene knockout strategy involves using a random mutagen (trimethylpsoralen) to mutagenize a very large number of worms. The worms are divided into many small subcultures and allowed to have progeny. A portion of each subculture is stored alive in the freezer, and genomic DNA is made from the rest of the culture.
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 839, Reviews: 0  Write review   Cached
  • C. elegans microinjection (Dr. Ian Chin-Sang)
    One way to make transgenic animals in C. elegans we use a microinjection technique. Briefly, a DNA construct (plasmid, cosmid or YAC) or PCR product with your gene(s) of interest is mixed with a co-injection marker and injected into the distal gonad (syncytium). The injected DNA is taken up into the mature oocyte's nucleus.
    http://lab.methodmint.com/methods/1441/
    Added: Thu Feb 16 2012, Hits: 20, Reviews: 0  Write review  
  • C. elegans single worm PCR (Dr. Ian Chin-Sang)
    Single Worm PCR protocol for use with C. elegans
    http://lab.methodmint.com/methods/1437/
    Added: Thu Feb 16 2012, Hits: 21, Reviews: 0  Write review  
  • Cleaning Worm Stocks (Michael Koelle) (Ambros Lab)
    The same protocol as above at another site.
    http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
    Added: Tue May 14 2002, Hits: 678, Reviews: 0  Write review   Cached
  • Cleaning worm strains (Michael Koelle's Lab)
    Clean fungi and bacteria contaminations.
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 686, Reviews: 0  Write review   Cached
  • Culturing Worms (Michael Koelle's Lab)
    Grow worms and prepare worm food.
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 1142, Reviews: 0  Write review   Cached
  • Dauer Pheromone Preparation: ( )
    MGH
    http://www.dartmouth.edu/artsci/bio/ambros/protoco...
    Added: Tue May 14 2002, Hits: 90, Reviews: 0  Write review   Cached
  • Development of techniques for primary culture of ltIgtC. eleganslt/Igt embryonic neurons (Laird Bloo (Ambros Lab)
    Although the techniques for cell culture developed in this section are not fully reproducible, the observation that axons can be grown from cultured C. elegans neurons is encouraging and suggests that further optimization should be attempted. The experiments described here were designed to extend these techniques to the growth of large numbers of dissociated embryonic cells. The goal was to define media, substrates, and cell isolation techniques that would permit neuronal differentiation and axonal outgrowth in short-term cultures...
    http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
    Added: Tue May 14 2002, Hits: 714, Reviews: 0  Write review   Cached
  • Egg laying in response to food (Michael Koelle's Lab)
    This method is used to determine how well worms regulate egg laying in response to food quantitatively.
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 626, Reviews: 0  Write review   Cached
  • Embryo Buffers (Aroian Lab)
    http://www-biology.ucsd.edu/labs/aroian/protocols/...
    Added: Tue May 14 2002, Hits: 85, Reviews: 0  Write review   Cached
  • EMS mutagenesis (Michael Koelle's Lab)
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 785, Reviews: 0  Write review   Cached
  • EMS Mutagenesis (Aroian Lab)
    http://www-biology.ucsd.edu/labs/aroian/protocols/...
    Added: Tue May 14 2002, Hits: 141, Reviews: 0  Write review   Cached
  • EMS Mutagenesis (Seydoux Lab)
    http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/EM...
    Added: Tue May 14 2002, Hits: 125, Reviews: 0  Write review   Cached
  • EMS mutagenesis (Herman Lab)
    http://www.ksu.edu/hermanlab/protocols/ems_mutagen...
    Added: Tue May 14 2002, Hits: 636, Reviews: 0  Write review   Cached
  • EMS Mutagenesis (Dr. Ian Chin-Sang)
    EMS Mutagenesis protocol
    http://lab.methodmint.com/methods/1424/
    Added: Mon Feb 06 2012, Hits: 15, Reviews: 0  Write review  
  • F2 mutant screen (Michael Koelle's Lab)
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 619, Reviews: 0  Write review   Cached
  • Fermentor (Herman Lab)
    http://www.ksu.edu/hermanlab/protocols/fermentor.h...
    Added: Tue May 14 2002, Hits: 530, Reviews: 0  Write review   Cached
  • Fixation & Staining Parameters (Aroian Lab)
    http://www-biology.ucsd.edu/labs/aroian/protocols/...
    Added: Tue May 14 2002, Hits: 101, Reviews: 0  Write review   Cached
  • Floating Worms on Sucrose (MGH) (Ambros Lab)
    Cleaning worms by floating on sucrose
    http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
    Added: Tue May 14 2002, Hits: 572, Reviews: 0  Write review   Cached
  • Freeze-fracture (Aroian Lab)
    http://www-biology.ucsd.edu/labs/aroian/protocols/...
    Added: Tue May 14 2002, Hits: 87, Reviews: 0  Write review   Cached
  • Freezing of Worm Stocks (Seydoux Lab)
    http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/Fr...
    Added: Tue May 14 2002, Hits: 103, Reviews: 0  Write review   Cached
  • Freezing Worms (Michael Koelle's Lab)
    Two procedures are introduced in this protocol with recipes.
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 608, Reviews: 0  Write review   Cached
  • Freezing worms (Michael Koelle's Lab)
    http://info.med.yale.edu/mbb/koelle/protocols/prot...
    Added: Tue May 14 2002, Hits: 562, Reviews: 0  Write review   Cached

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