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Intracellular Cytokine Staining

Intracellular Cytokine Staining

Staining Protocols &
Ordering Information:

Intracellular Cytokine

Mouse Cell Surface

Human Cell Surface


Quick Guide Chart for Intracellular Staining:

eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified, FITC, Cy5, PE, PE-Cy5, and APC conjugates. For more information about the properties of fluorescent dyes, please visit our Fluorescent Dyes page in our BestProtocols. The Functional Grade Purified format is recommended for neutralization studies. More antibodies are in development; if you do not see an antibody of your choice, please send us an e-mail so we may provide you timely product updates.

 

Cytokine

Source

Activation

Time

Restimulation

Block

Antibody

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

mIL-2

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

JES6-5H4

mIL-4

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

11B11

mIL-6

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

MP5-20F3

mIL-10

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

JES5-16E3

mGM-CSF

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

MP1-22E9

mIFN-g

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

XMG1.2

mTNF-a

mouse
spleen

1) ConA (3µg/ml) for two days

2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days

2d/3d

anti-CD3 (10µg/ml immobilized) +

anti-CD28 (2µg/ml soluble) + monensin for 5hrs

Monensin

MP6-XT22