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Protocols
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Antigen Design & Sera Purification
(Sigma)
This protocol describes topics such as peptide selection and design, coupling strategy, selecting the protein carrier, multiple antigenic peptides (MAPs), choice of host, adjuvant, immunization, & sera collection, antisera purification, ammonium sulfate precipitation
protein A/G, and immunoaffinity purification
http://www.sigmaaldrich.com/Brands/Sigma_Genosys/C...
Added: Tue May 14 2002, Hits: 3349, Reviews: 0
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Preparation Of Peptide-KLH Conjugates For Immunization
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/ab1.htm...
Added: Tue May 14 2002, Hits: 1227, Reviews: 0
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Testing Rabbit Bleeds By Elisa
(Mitchison Lab)
You can test whether or not you have gotten an immune response to the peptide and how strong that immune response is by doing ELISAs against peptide conjugated to BSA.
http://mitchison.med.harvard.edu/protocols/ab2.htm...
Added: Tue May 14 2002, Hits: 1062, Reviews: 0
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