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Protocols
- Polyclonal Antibody Production (Kitto Lab, The University of Texas at Austin)
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.
http://research.cm.utexas.edu/bkitto/Kittolabpage/...
Added: Tue Oct 08 2002, Hits: 7454, Reviews: 0 Write review Cached -
Coupling Peptides to Resin for Affinity Purification
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab3.htm...
Added: Tue May 14 2002, Hits: 1200, Reviews: 0 Write review Cached -
GST-fusion protein injection into rabbits
(IVAN J. DELGADO ORLIC)
http://www.msu.edu/~delgadoi/154.html
Added: Tue May 14 2002, Hits: 1452, Reviews: 0 Write review Cached -
How Do I make an Antibody against a Peptides
(Pierce)
http://www.piercenet.com/y/production/producebotto...
Added: Tue May 14 2002, Hits: 3139, Reviews: 0 Write review Cached -
Making antibodies to synthetic peptides
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
http://medicine.ucsd.edu/hypertension/list_of_prot...
Added: Tue May 14 2002, Hits: 1710, Reviews: 0 Write review Cached -
Preparation of Peptide-KLH Conjugates for Immunization
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab1.htm...
Added: Tue May 14 2002, Hits: 869, Reviews: 0 Write review Cached -
Preparation of Polyclonal Antibodies
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Immunization and antisera preparation
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 2429, Reviews: 0 Write review Cached -
Preparation of Site-Specific Histone Antibodies and Affinity Purifications
(Chromatin and Gene Expression Group, The University of Birmingham, UK)
Provides guidelines for preparation of polyclonal antibodies against histones, including steps from synthesis of N-terminal peptides of histones H2A, H2B, H3 and H4, conjugation of synthetic peptides to ovalbumin, immunization of rabbits, evaluation of the antibody response to affinity chromatography.
http://medweb.bham.ac.uk/research/chromatin/protoc...
Added: Sun Jan 08 2006, Hits: 639, Reviews: 0 Write review Cached -
Purification of Anti Peptide Antibody
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab4.htm...
Added: Tue May 14 2002, Hits: 1782, Reviews: 0 Write review Cached -
Testing Rabbit Bleeds by Elisa
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab2.htm...
Added: Tue May 14 2002, Hits: 1428, Reviews: 0 Write review Cached