Protocols

• BAC DNA Purification for Microinjection (University of Michigan, Transgenic Animal Model Core)
  This method is used to purify BAC DNA for microinjection into fertilized mouse eggs. This method yields clean, non-sheared DNA
  https://www.med.umich.edu/tamc/BACDNA.html
  Added: Tue May 14 2002, Hits: 160, Reviews: 0  Write review   Cached
• DNA Preparation for microinjection or electroporation (Transgenic and ES Cell Services at OSU)
  https://www.med.ohio-state.edu/tafhome/webpages/pro...
  Added: Tue May 14 2002, Hits: 138, Reviews: 0  Write review   Cached
• DNA Purification for Electroporation (University of Michigan, Transgenic Animal Model Core)
  https://www.med.umich.edu/tamc/electro.html
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• DNA Purification for Microinjection (University of Michigan, Transgenic Animal Model Core)
  https://www.med.umich.edu/tamc/injDNA.html
  Added: Tue May 14 2002, Hits: 91, Reviews: 0  Write review   Cached
• DNA Purification for Transgene Microinjection (U. of Virginia Transgenic Mouse Core Facility)
  https://www.med.virginia.edu/medicine/inter-dis/tra...
  Added: Tue May 14 2002, Hits: 77, Reviews: 0  Write review   Cached
• DNA Ultrapurification for Microinjection (Gary A. J. Brown)
  Several methods are described in detail.
  https://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 58, Reviews: 0  Write review   Cached
• Isolation of genomic DNA from tail biopsies to check for transgenic founder animals (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-9-8.pdf
  Added: Wed Sep 04 2002, Hits: 65, Reviews: 0  Write review  
• Isolation of linearized BAC DNA for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-9-3.pdf
  Added: Wed Sep 04 2002, Hits: 69, Reviews: 0  Write review  
• Isolation of linearized plasmid DNA constructs for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-9-1.pdf
  Added: Wed Sep 04 2002, Hits: 69, Reviews: 0  Write review  
• Isolation of YAC DNA for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  https://www.oup.co.uk/pdf/pas/13-9-2.pdf
  Added: Wed Sep 04 2002, Hits: 34, Reviews: 0  Write review  
• Preparation of DNA for Microinjection (Pharmacological Sciences Consortium Core)
  Restrict DNA, gel purification, ethanol precipitation...
  https://transgenic.cwru.edu/DNA.htm
  Added: Tue May 14 2002, Hits: 82, Reviews: 0  Write review   Cached
• Preparation of Transgene DNA for Microinjection (New England Medical Center)
  https://www.nemc.org/mcri/transgenicservices/guidel...
  Added: Tue May 14 2002, Hits: 70, Reviews: 0  Write review   Cached
• Sample Preparation for Microinjection (Brinkmann)
  Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm [1]. This capillary is back loaded with the substance to be transferred into the cells cultured for microinjection. Typical substances include purified antibodies, DNA, RNA, peptides, or oligonucleotides.
  https://www.brinkmann.com/ECET_appl2.asp
  Added: Tue May 14 2002, Hits: 67, Reviews: 0  Write review   Cached

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